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验证数据展示

  • IF Staining of A431 using 66632-1-Ig (same clone as 66632-1-PBS)

    IF Staining of A431 using 66632-1-Ig (same clone as 66632-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NEK2 antibody (66632-1-Ig, Clone: 3D7B4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

  • WB analysis of human peripheral blood leukocyte using 66632-1-Ig (same clone as 66632-1-PBS)

    WB analysis of human peripheral blood leukocyte using 66632-1-Ig (same clone as 66632-1-PBS)

    Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66632-1-Ig (same clone as 66632-1-PBS)

    IHC staining of human testis using 66632-1-Ig (same clone as 66632-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66632-1-Ig (NEK2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NEK2 antibody included
  • IHC staining of human testis using 66632-1-Ig (same clone as 66632-1-PBS)

    IHC staining of human testis using 66632-1-Ig (same clone as 66632-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66632-1-Ig (NEK2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NEK2 antibody included

NEK2 Monoclonal antibody, PBS Only

NEK2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66632-1-PBS

产品说明书

CloneNo. 3D7B4

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse , Rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NEK2 Ready-To-Use IHC Kit. NEK2 primary antibody included.

产品信息

66632-1-PBS targets NEK2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse , Rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse , Rat
免疫原

CatNo: Ag21476

Product name: Recombinant human NEK2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-445 aa of BC043502

Sequence: MPSRAEDYEVLYTIGTGSYGRCQKIRRKSDGKILVWKELDYGSMTEAEKQMLVSEVNLLRELKHPNIVRYYDRIIDRTNTTLYIVMEYCEGGDLASVITKGTKERQYLDEEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLDGKQNVKLGDFGLARILNHDTSFAKTFVGTPYYMSPEQMNRMSYNEKSDIWSLGCLLYELCALMPPFTAFSQKELAGKIREGKFRRIPYRYSDELNEIITRMLNLKDYHRPSVEEILENPLIADLVADEQRRNLERRGRQLGEPEKSQDSSPVLSELKLKEIQLQERERALKAREERLEQKEQELCVRERLAEDKLARAENLLKNYSLLKERKFLSLASNPELLNLPSSVIKKKVHFSGESKENIMRSENSESQLTSKSKCKDLKKRLHAAQLRAQALSDIEKNYQLKSRQILGMR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 NIMA (never in mitosis gene a)-related kinase 2
别名 HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1
计算分子量 52 kDa
观测分子量 48 kDa
GenBank蛋白编号BC043502
基因名称 NEK2
Gene ID (NCBI) 4751
RRIDAB_2881991
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP51955
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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