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NEIL3 Polyclonal antibody

NEIL3 Polyclonal Antibody for WB, ELISA
Cat No. 11621-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA and More (2)

DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Endonuclease 8 like 3, Endonuclease VIII like 3, FGP2, FPG2, hFPG2, hNEI3, Nei like protein 3, NEI3, NEIL3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, mouse skin tissue, human skin tissue, THP-1 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11621-1-AP targets NEIL3 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IP, ChIP
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag2200

Product name: Recombinant human NEIL3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 257-605 aa of BC025954

Sequence: CHCRITVCRFGDNNRMTYFCPHCQKENPQHVDICKLPTRNTIISWTSSRVDHVMDSVARKSEEHWTCVVCTLINKPSSKACDACLTSRPIDSVLKSEENSTVFSHLMKYPCNTFGKPHTEVKINRKTAFGTTTLVLTDFSNKSSTLERKTKQNQILDEEFQNSPPASVCLNDIQHPSKKTTNDITQLSSKVNISPTISSESKLFSPAHKKPKTAHYSSPELKSCNPGYSNSELQINMTDGPRTLNPDSPRCSKHNRLCILRVVRKDGENKGRQFYACPLPREAQCGFFEWADLSFPFCNHGKRSTMKTVLKIGPNNGKNFFVCPLGKEKQCNFFQWAENGPGIKIIPGC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nei endonuclease VIII-like 3 (E. coli)
别名 DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Endonuclease 8 like 3, Endonuclease VIII like 3, FGP2, FPG2, hFPG2, hNEI3, Nei like protein 3, NEI3, NEIL3
计算分子量 605 aa, 68 kDa
观测分子量 62-68 kDa
GenBank蛋白编号BC025954
基因名称 NEIL3
Gene ID (NCBI) 55247
RRIDAB_2251229
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TAT5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEIL3 belongs to the FPG family. It is a functional DNA glycosylase in vitro and in vivo. NEIL3 plays a role in repairing FapyG in vivo. (PMID:20185759) NEIL3 has AP lyase activity specific for single-stranded DNA.(PMID:19170771). NEIL3 is specifically expressed in brain areas where neurogenesis takes place during development and that its expression is tightly regulated both temporally and spatially (PMID:19426544). Studies have shown that NEIL3 is recruited to the DSB sites through its GRF zinc finger motifs and plays an important role in MMC/cisplatin-ICL repair by promoting the HR step in FA/BRCA pathway.(PMID: 36351389).

实验方案

Product Specific Protocols
WB protocol for NEIL3 antibody 11621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation.

Authors - Dan Liu
humanWB

Nucleic Acids Res

Cooperation of the NEIL3 and Fanconi anemia/BRCA pathways in interstrand crosslink repair.

Authors - Niu Li
  • KD Validated
humanWB

EMBO J

PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks.

Authors - Daniel González-Acosta
humanWB

Sci Rep

NEIL3-mediated proteasomal degradation facilitates the repair of cisplatin-induced DNA damage in human cells

Authors - Umit Aliyaskarova
humanWB,IP

DNA Repair (Amst)

Release from quiescence stimulates the expression of human NEIL3 under the control of the Ras dependent ERK-MAP kinase pathway.

Authors - Neurauter Christine Gran CG
humanWB,FC,IP,ChIP

Cell Rep

NEIL3 Repairs Telomere Damage during S Phase to Secure Chromosome Segregation at Mitosis.

Authors - Jia Zhou
  • KD Validated
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