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验证数据展示

  • IHC staining of mouse brain using 12998-1-AP

    IHC staining of mouse brain using 12998-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12998-1-AP (NF-L antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NF-L antibody included
  • IHC staining of mouse brain using 12998-1-AP

    IHC staining of mouse brain using 12998-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12998-1-AP (NF-L antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NF-L antibody included
  • WB analysis of mouse brain tissue using 12998-1-AP

    WB analysis of mouse brain tissue using 12998-1-AP

    WB result of 12998-1-AP.

  • FC experiment of SH-SY5Y using 12998-1-AP

    FC experiment of SH-SY5Y using 12998-1-AP

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human NF-L (12998-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • WB analysis using 12998-1-AP

    WB analysis using 12998-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12998-1-AP (NF-L antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of SH-SY5Y using 12998-1-AP

    IP experiment of SH-SY5Y using 12998-1-AP

    IP result of anti-NF-L (IP:12998-1-AP, 4ug; Detection:12998-1-AP 1:40000) with SH-SY5Y cells lysate 880 ug.

  • IF Staining of mouse brain using 12998-1-AP

    IF Staining of mouse brain using 12998-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NF-L antibody (12998-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of rat brain using 12998-1-AP

    IF Staining of rat brain using 12998-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using NF-L antibody (12998-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of mouse brain using 12998-1-AP

    IF Staining of mouse brain using 12998-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using NF-L antibody (12998-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of SH-SY5Y using 12998-1-AP

    IF Staining of SH-SY5Y using 12998-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using NF-L antibody (12998-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of SH-SY5Y using 12998-1-AP

    IF Staining of SH-SY5Y using 12998-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NF-L antibody (12998-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

NF-L Polyclonal antibody

NF-L Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA
Cat No. 12998-1-AP

产品说明书

引用文献(36)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA and More (1)

别名

NEFL, neurofilament, NF68, NFL

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, Neuro-2a cells, mouse brain tissue, C6 cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF-Fro detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy NF-L Ready-To-Use IHC Kit. NF-L primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12998-1-AP targets NF-L in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, ELISA
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, monkey
免疫原

CatNo: Ag3667

Product name: Recombinant human NEFL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-352 aa of BC039237

Sequence: MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTIN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neurofilament, light polypeptide
别名 NEFL, neurofilament, NF68, NFL
计算分子量 543 aa, 62 kDa
观测分子量 65-68 kDa, 140-160 kDa
GenBank蛋白编号BC039237
基因名称 NF-L
Gene ID (NCBI) 4747
RRIDAB_10597388
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07196
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M, 145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL. It has a very weak reaction to NEFM.

实验方案

Product Specific Protocols
FC protocol for NF-L antibody 12998-1-APDownload protocol
IF protocol for NF-L antibody 12998-1-APDownload protocol
IHC protocol for NF-L antibody 12998-1-APDownload protocol
IP protocol for NF-L antibody 12998-1-APDownload protocol
WB protocol for NF-L antibody 12998-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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