验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells, MCF-7 cells, U-251 cells, Raji cells, Jurkat cells |
| Positive IF/ICC detected in | MCF-7 cells, A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83362-2-RR targets NEDD9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag34170 Product name: Recombinant human NEDD9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 227-458 aa of BC040207 Sequence: PSACRDEAGLREKDYDFPPPMRQAGRPDLRPEGVYDIPPTCTKPAGKDLHVKYNCDIPGAAEPVARRHQSLSPNHPPPQLGQSVGSQNDAYDVPRGVQFLEPPAETSEKANPQERDGVYDVPLHNPPDAKGSRDLVDGINRLSFSSTGSTRSNMSTSSTSSKESSLSASPAQDKRLFLDPDTAIERLQRLQQALEMGVSSLMALVTTDWRCYGYMERHINEIRTAVDKVELF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | neural precursor cell expressed, developmentally down-regulated 9 |
| 别名 | HEF1, Enhancer of filamentation 1 p55, CRK-associated substrate-related protein, CAS-L, CASL |
| 计算分子量 | 834 aa, 93 kDa |
| 观测分子量 | 105 kDa, 115 kDa |
| GenBank蛋白编号 | BC040207 |
| 基因名称 | NEDD9 |
| Gene ID (NCBI) | 4739 |
| RRID | AB_3671016 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q14511 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NEDD9, also known as CASL, HEF1, contains an N-terminal Src homology (SH) 3 domain and an adjacent domain composed of multiple SH2-binding motifs(PMID: 8668148). Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55. Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle. Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. p105 and p115 are predominantly cytoplasmic and associated with focal adhesions, whereas p55associates with the mitotic spindle(PMID: 9584194).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NEDD9 antibody 83362-2-RR | Download protocol |
| WB protocol for NEDD9 antibody 83362-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
