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NEDD4 Recombinant antibody

NEDD4 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230478F1

Cat No : 83112-1-RR

Print datasheet

Synonyms

KIAA0093, NEDD 4, NEDD4, NEDD4 1, RPF1

验证数据展示

  • WB analysis using 83112-1-RR

    WB analysis using 83112-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83112-1-RR (NEDD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inC6 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83112-1-RR targets NEDD4 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen NEDD4 fusion protein Ag16291 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name neural precursor cell expressed, developmentally down-regulated 4
Synonyms KIAA0093, NEDD 4, NEDD4, NEDD4 1, RPF1
Calculated Molecular Weight 1319 aa, 149 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC152452
Gene Symbol NEDD4
Gene ID (NCBI) 4734
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP46934
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDL1 and NEDL2. NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain. NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).

实验方案

Product Specific Protocols
WB protocol for NEDD4 antibody 83112-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols