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验证数据展示

  • WB analysis of human brain using 18940-1-AP

    WB analysis of human brain using 18940-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18940-1-AP (NECAB3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 18940-1-AP

    IHC staining of human pancreas using 18940-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18940-1-AP (NECAB3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human pancreas using 18940-1-AP

    IHC staining of human pancreas using 18940-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18940-1-AP (NECAB3 antibody) at dilution of 1:100 (under 40x lens).

NECAB3 Polyclonal antibody

NECAB3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18940-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

APBA2BP, EFCBP3, NECAB3, Nek2 interacting protein 1, NIP1, STIP3, SYTIP2, X11L binding protein 51, XB51

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

18940-1-AP targets NECAB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag13493

Product name: Recombinant human NECAB3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-362 aa of BC047673

Sequence: MACAGLLTVCLLRPPAPQPQPQTPRHPQLAPDPGPAGHTLFQDVFRRADKNDDGKLSFEEFQNYFADGVLSLGELQELFSGIDGHLTDNLETEKLCDYFSEHLGVYRPVLAALESLNRAVLAAMDATKLEYERASKVDQFVTRFLLRETVSQLQALQSSLEGASDTLEAQAHGWRSDAESVEAQSRLCGSRRAGRRALRSVSRSSTWSPGSSDTGRSSEAEMQWRLQVNRLQELIDQLECKAPRLEPLREEDLAKGPDLHILMAQRQVQVAEEGLQDFHRALRCYVDFTGAQSHCLHVSAQKMLDGASFTLYEFWQDEASWRRHQQSPGSKAFQRILIDHLRAPDTLTTVFFPASWWIMNNN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-terminal EF-hand calcium binding protein 3
别名 APBA2BP, EFCBP3, NECAB3, Nek2 interacting protein 1, NIP1, STIP3, SYTIP2, X11L binding protein 51, XB51
计算分子量362aa,41 kDa; 396aa,44 kDa
观测分子量 40-44 kDa
GenBank蛋白编号BC047673
基因名称 NECAB3
Gene ID (NCBI) 63941
RRIDAB_10596622
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96P71
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NECAB3, also named as APBA2BP, NIP1, SYTIP2 and XB51, is a potential substrate of Nek2. NECAB3 inhibits the interaction of APBA2 with beta-amyloid precursor protein (APP). NECAB3 has three isoforms with MW 40 kDa, 44 kDa and 22 kDa.

实验方案

Product Specific Protocols
WB protocol for NECAB3 antibody 18940-1-APDownload protocol
IHC protocol for NECAB3 antibody 18940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

PLoS One

Amyloidosis, Synucleinopathy, and Prion Encephalopathy in a Neuropathic Lysosomal Storage Disease: The CNS-Biomarker Potential of Peripheral Blood.

Authors - Naughton Bartholomew J BJ
View Article