验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, rat brain tissue |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
18940-1-AP targets NECAB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag13493 Product name: Recombinant human NECAB3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-362 aa of BC047673 Sequence: MACAGLLTVCLLRPPAPQPQPQTPRHPQLAPDPGPAGHTLFQDVFRRADKNDDGKLSFEEFQNYFADGVLSLGELQELFSGIDGHLTDNLETEKLCDYFSEHLGVYRPVLAALESLNRAVLAAMDATKLEYERASKVDQFVTRFLLRETVSQLQALQSSLEGASDTLEAQAHGWRSDAESVEAQSRLCGSRRAGRRALRSVSRSSTWSPGSSDTGRSSEAEMQWRLQVNRLQELIDQLECKAPRLEPLREEDLAKGPDLHILMAQRQVQVAEEGLQDFHRALRCYVDFTGAQSHCLHVSAQKMLDGASFTLYEFWQDEASWRRHQQSPGSKAFQRILIDHLRAPDTLTTVFFPASWWIMNNN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | N-terminal EF-hand calcium binding protein 3 |
| 别名 | APBA2BP, EFCBP3, NECAB3, Nek2 interacting protein 1, NIP1, STIP3, SYTIP2, X11L binding protein 51, XB51 |
| 计算分子量 | 362aa,41 kDa; 396aa,44 kDa |
| 观测分子量 | 40-44 kDa |
| GenBank蛋白编号 | BC047673 |
| 基因名称 | NECAB3 |
| Gene ID (NCBI) | 63941 |
| RRID | AB_10596622 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96P71 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NECAB3, also named as APBA2BP, NIP1, SYTIP2 and XB51, is a potential substrate of Nek2. NECAB3 inhibits the interaction of APBA2 with beta-amyloid precursor protein (APP). NECAB3 has three isoforms with MW 40 kDa, 44 kDa and 22 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NECAB3 antibody 18940-1-AP | Download protocol |
| WB protocol for NECAB3 antibody 18940-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
