验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, human heart tissue, human liver tissue |
| Positive IHC detected in | human placenta tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
13430-1-AP targets NDUFV3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag4234 Product name: Recombinant human NDUFV3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-108 aa of BC033766 Sequence: MAAPCLLRQGRAGALKTMLQEAQVFRGLASTVSLSAESGKSEKGQPQNSKKQSPPKKPAPVPAEPFDNTTYKNLQHHDYSTYTFLDLNLELSKFRMPQPSSGRESPRH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NADH dehydrogenase (ubiquinone) flavoprotein 3, 10kDa |
| 别名 | Renal carcinoma antigen NY-REN-4, NADH-ubiquinone oxidoreductase 9 kDa subunit, Complex I-9kD, Complex I 9kD, CI-9kD |
| 计算分子量 | 108 aa, 12 kDa |
| 观测分子量 | 10 kDa |
| GenBank蛋白编号 | BC033766 |
| 基因名称 | NDUFV3 |
| Gene ID (NCBI) | 4731 |
| RRID | AB_2282462 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P56181 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NDUFV3(NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial) is also named as CI-9kD,Renal carcinoma antigen NY-REN-4 and belongs to the complex I NDUFV3 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NDUFV3 antibody 13430-1-AP | Download protocol |
| IHC protocol for NDUFV3 antibody 13430-1-AP | Download protocol |
| WB protocol for NDUFV3 antibody 13430-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Proteome Res Quantitative proteomics reveals oxygen-dependent changes in neuronal mitochondria affecting function and sensitivity to rotenone. | ||
J Pharmacol Exp Ther Metabolic Mechanisms and a Rational Combinational Application of Carboxyamidotriazole in Fighting Pancreatic Cancer Progression after Chemotherapy. | ||
F1000Res Androgen-dependent alternative mRNA isoform expression in prostate cancer cells. | ||
