NDUFV3 Polyclonal antibody
NDUFV3 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, human heart tissue, human liver tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
13430-1-AP targets NDUFV3 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | NDUFV3 fusion protein Ag4234 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | NADH dehydrogenase (ubiquinone) flavoprotein 3, 10kDa |
| Synonyms | CI 9KD, Complex I 9kD, NDUFV3 |
| Calculated Molecular Weight | 108 aa, 12 kDa |
| Observed Molecular Weight | 10 kDa |
| GenBank Accession Number | BC033766 |
| Gene Symbol | NDUFV3 |
| Gene ID (NCBI) | 4731 |
| RRID | AB_2282462 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P56181 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NDUFV3(NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial) is also named as CI-9kD,Renal carcinoma antigen NY-REN-4 and belongs to the complex I NDUFV3 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NDUFV3 antibody 13430-1-AP | Download protocol |
| IHC protocol for NDUFV3 antibody 13430-1-AP | Download protocol |
| IF protocol for NDUFV3 antibody 13430-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Proteome Res Quantitative proteomics reveals oxygen-dependent changes in neuronal mitochondria affecting function and sensitivity to rotenone. | ||
J Pharmacol Exp Ther Metabolic Mechanisms and a Rational Combinational Application of Carboxyamidotriazole in Fighting Pancreatic Cancer Progression after Chemotherapy. | ||
F1000Res Androgen-dependent alternative mRNA isoform expression in prostate cancer cells. |
