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验证数据展示

  • WB analysis using 25172-1-AP

    WB analysis using 25172-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25172-1-AP (NDUFS8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 25172-1-AP

    IHC staining of human liver cancer using 25172-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25172-1-AP (NDUFS8 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 25172-1-AP

    IHC staining of human liver cancer using 25172-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25172-1-AP (NDUFS8 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of A431 using 25172-1-AP

    IF Staining of A431 using 25172-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NDUFS8 antibody (25172-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NDUFS8 Polyclonal antibody

NDUFS8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25172-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

CI 23kD, CI-23kD, Complex I 23kD, Complex I-23kD, EC:7.1.1.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA2780 cells, SKOV-3 cells, mouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25172-1-AP targets NDUFS8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原

CatNo: Ag18531

Product name: Recombinant human NDUFS8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-210 aa of BC119754

Sequence: MRCLTTPMLLRALAQAARAGPPGGRSLHSSAVAATYKYVNMQDPEMDMKSVTDRAARTLLWTELFRGLGMTLSYLFREPATINYPFEKGPLSPRFRGEHALRRYPSGEERCIACKLCEAICPAQAITIEAEPRADGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFSTETHEELLYNKEKLLNNGDKWEAEIAANIQADYLYR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase)
别名 CI 23kD, CI-23kD, Complex I 23kD, Complex I-23kD, EC:7.1.1.2
计算分子量 210 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC119754
基因名称 NDUFS8
Gene ID (NCBI) 4728
RRIDAB_2879939
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00217
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NADH: ubiquinone oxidoreductase core subunit S8 (NDUFS8), also called TYKY, is an essential core subunit and component of the iron-sulfur (FeS) fragment of mitochondrial complex I directly involved in the electron transfer process and energy metabolism (PMID: 36557887). Pathogenic variants of the NDUFS8 are relevant to infantile-onset and severe diseases, including Leigh syndrome, cancer, and diabetes mellitus (PMID: 36557887). Knockdown of NDUFS8 impaired mitochondrial function, increased ROS, disrupted redox homeostasis, induced apoptosis, and suppressed proliferation and migration of HCC cells (PMID: 40914145).

实验方案

Product Specific Protocols
WB protocol for NDUFS8 antibody 25172-1-APDownload protocol
IHC protocol for NDUFS8 antibody 25172-1-APDownload protocol
IF protocol for NDUFS8 antibody 25172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Biomed Pharmacother

Combination of RNA-seq and proteomics reveals the mechanism of DL0410 treatment in APP/PS1 transgenic mouse model of Alzheimer's disease

Authors - Baoyue Zhang
View Article