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验证数据展示

  • WB analysis using 14417-1-AP (same clone as 14417-1-PBS)

    WB analysis using 14417-1-AP (same clone as 14417-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 14417-1-AP (same clone as 14417-1-PBS)

    IP experiment of mouse liver using 14417-1-AP (same clone as 14417-1-PBS)

    IP result of anti-NDUFS6 (IP:14417-1-AP, 4ug; Detection:14417-1-AP 1:300) with mouse liver tissue lysate 3200 ug. This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 14417-1-AP (same clone as 14417-1-PBS)

    IF Staining of HepG2 using 14417-1-AP (same clone as 14417-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS6 antibody (14417-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 14417-1-AP (same clone as 14417-1-PBS)

    IF Staining of HeLa using 14417-1-AP (same clone as 14417-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFS6 antibody (14417-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 14417-1-AP (same clone as 14417-1-PBS)

    IHC staining of mouse brain using 14417-1-AP (same clone as 14417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14417-1-AP (NDUFS6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFS6 antibody included
  • IHC staining of mouse brain using 14417-1-AP (same clone as 14417-1-PBS)

    IHC staining of mouse brain using 14417-1-AP (same clone as 14417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14417-1-AP (NDUFS6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFS6 antibody included

NDUFS6 Polyclonal antibody, PBS Only

NDUFS6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14417-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

CI 13kD A, CI-13kD-A, Complex I 13kD A, Complex I-13kD-A, NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® NDUFS6 Ready-To-Use IHC Kit. NDUFS6 primary antibody included.

产品信息

14417-1-PBS targets NDUFS6 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5762

Product name: Recombinant human NDUFS6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-124 aa of BC046155

Sequence: MAAAMTFCRLLNRCGEAARSLPLGARCFGVRVSPTGEKVTHTGQVYDDKDYRRIRFVGRQKEVNENFAIDLIAEQPVSEVETRVIACDGGGGALGHPKVYINLDKETKTGTCGYCGLQFRQHHH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Q reductase)
别名 CI 13kD A, CI-13kD-A, Complex I 13kD A, Complex I-13kD-A, NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
计算分子量 14 kDa
观测分子量 13 kDa
GenBank蛋白编号BC046155
基因名称 NDUFS6
Gene ID (NCBI) 4726
RRIDAB_2149024
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75380
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDUFS6(NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial) is also named as 1CI-13kD-A, NADH-ubiquinone oxidoreductase 13 kDa-A subunit and belongs to the complex I NDUFS6 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.

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