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NDUFC2 Polyclonal antibody

NDUFC2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 15573-1-AP

Print datasheet

Synonyms

B14.5b, CI B14.5b, Complex I B14.5b, HLC 1, NADHDH2, NDUFC2

验证数据展示

  • WB analysis using 15573-1-AP

    WB analysis using 15573-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15573-1-AP (NDUFC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15573-1-AP

    IHC staining of human kidney using 15573-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15573-1-AP (NDUFC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 15573-1-AP

    IHC staining of human kidney using 15573-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15573-1-AP (NDUFC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, human liver tissue, human kidney tissue, Hela cells, HepG2 cells
Positive IHC detected inhuman kidney tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

The immunogen of 15573-1-AP is NDUFC2 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Immunogen NDUFC2 fusion protein Ag7919 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa
Synonyms B14.5b, CI B14.5b, Complex I B14.5b, HLC 1, NADHDH2, NDUFC2
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC007323
Gene Symbol NDUFC2
Gene ID (NCBI) 4718
RRIDAB_10666733
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95298
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NDUFC2 antibody 15573-1-APDownload protocol
IHC protocol for NDUFC2 antibody 15573-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Intellectual disability-associated factor Zbtb11 cooperates with NRF-2/GABP to control mitochondrial function.

Authors - Brooke C Wilson
View Article
pigWB

Sci Rep

Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs.

Authors - Liangliang Fu
View Article
humanWB

Cell Rep

A membrane arm of mitochondrial complex I sufficient to promote respirasome formation.

Authors - Hezhi Fang
View Article