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验证数据展示

  • WB analysis using RMX00097

    WB analysis using RMX00097

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00097 (NDUFB8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using RMX00097

    IHC staining of mouse brain using RMX00097

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00097 (NDUFB8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFB8 antibody included
  • IF Staining of HepG2 using RMX00097

    IF Staining of HepG2 using RMX00097

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (RMX00097) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Multi-rAb® NDUFB8 Multi-Recombinant antibody

NDUFB8 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00097

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ASHI, CI ASHI, CIASHI, CI-ASHI, Complex I-ASHI

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy® NDUFB8 Ready-To-Use IHC Kit. NDUFB8 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00097 targets NDUFB8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
别名 ASHI, CI ASHI, CIASHI, CI-ASHI, Complex I-ASHI
计算分子量 22 kDa
观测分子量 19-22 kDa
GenBank蛋白编号BC000466
基因名称 NDUFB8
Gene ID (NCBI) 4714
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDO95169
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDUFS8 is encoded by a nuclear gene and is a member of the respiratory chain complex I. Respiratory chain complex I (CI; NADH: ubiquinone oxidoreductase), one of the largest membrane-bound protein complexes in the human cell, is essential for oxidative energy metabolism as it powers ATP synthesis by using the reducing potential of NADH to proton translocation across the inner mitochondrial membrane.

实验方案

Product Specific Protocols
IF protocol for NDUFB8 antibody RMX00097Download protocol
IHC protocol for NDUFB8 antibody RMX00097Download protocol
WB protocol for NDUFB8 antibody RMX00097Download protocol
Standard Protocols
Click here to view our Standard Protocols
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