验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, LNCaP cells, MCF-7 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16037-1-AP targets NDUFB6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | NDUFB6 fusion protein Ag8908 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa |
| 别名 | B17, CI, CI B17, Complex I B17, NDUFB6 |
| 计算分子量 | 128 aa, 15 kDa |
| 观测分子量 | 16-20 kDa |
| GenBank蛋白编号 | BC009801 |
| 基因名称 | NDUFB6 |
| Gene ID (NCBI) | 4712 |
| RRID | AB_2235901 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95139 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NDUFB6, also named as NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 or Complex I-B17, is a 128 amino acid protein, which belongs to the complex I NDUFB6 subunit family. NDUFB6 localizes in the Mitochondrion inner membrane and is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NDUFB6 antibody 16037-1-AP | Download protocol |
| IHC protocol for NDUFB6 antibody 16037-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Rep OMA1 reprograms metabolism under hypoxia to promote colorectal cancer development.
| ||
Redox Biol Mitochondrial complex I deactivation is related to superoxide production in acute hypoxia. | ||
J Nutr Biochem Deficiency of β-carotene oxygenase 2 induces mitochondrial fragmentation and activates the STING-IRF3 pathway in the mouse hypothalamus. | ||
Mitochondrion MNRR1 (formerly CHCHD2) is a bi-organellar regulator of mitochondrial metabolism. | ||
FEBS Lett Apoptosis-inducing factor is a target gene of C/EBPα and participates in adipocyte differentiation. |