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验证数据展示

  • WB analysis of mouse heart using 27931-1-AP

    WB analysis of mouse heart using 27931-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 27931-1-AP (NDUFB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 27931-1-AP

    IHC staining of human stomach cancer using 27931-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27931-1-AP (NDUFB4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFB4 antibody included
  • IHC staining of mouse skeletal muscle using 27931-1-AP

    IHC staining of mouse skeletal muscle using 27931-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27931-1-AP (NDUFB4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFB4 antibody included
  • IHC staining of human stomach cancer using 27931-1-AP

    IHC staining of human stomach cancer using 27931-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27931-1-AP (NDUFB4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFB4 antibody included
  • IHC staining of mouse stomach using 27931-1-AP

    IHC staining of mouse stomach using 27931-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 27931-1-AP (NDUFB4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFB4 antibody included
  • IHC staining of rat cerebellum using 27931-1-AP

    IHC staining of rat cerebellum using 27931-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 27931-1-AP (NDUFB4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFB4 antibody included
  • IHC staining of rat stomach using 27931-1-AP

    IHC staining of rat stomach using 27931-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 27931-1-AP (NDUFB4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NDUFB4 antibody included
  • IF Staining of HeLa using 27931-1-AP

    IF Staining of HeLa using 27931-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFB4 antibody (27931-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

NDUFB4 Polyclonal antibody

NDUFB4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27931-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

B15, CI B15, CI-B15, Complex I B15, Complex I-B15

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman stomach cancer tissue, mouse stomach tissue, rat cerebellum tissue, mouse skeletal muscle tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy® NDUFB4 Ready-To-Use IHC Kit. NDUFB4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

27931-1-AP targets NDUFB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag27484

Product name: Recombinant human NDUFB4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-128 aa of BC000855

Sequence: MSFPKYKPSSLRTLPETLDPAEYNISPETRRAQAERLAIRAQLKREYLLQYNDPNRRGLIENPALLRWAYARTINVYPNFRPTPKNSLMGALCGFGPLIFIYYIIKTERDRKEKLIQEGKLDRTFHLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa
别名 B15, CI B15, CI-B15, Complex I B15, Complex I-B15
计算分子量 15 kDa
观测分子量 15 kDa
GenBank蛋白编号BC000855
基因名称 NDUFB4
Gene ID (NCBI) 4710
RRIDAB_2881012
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95168
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for NDUFB4 antibody 27931-1-APDownload protocol
IHC protocol for NDUFB4 antibody 27931-1-APDownload protocol
WB protocol for NDUFB4 antibody 27931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cells

Metformin Induces Apoptosis and Ferroptosis of Ovarian Cancer Cells Under Energy Stress Conditions

Authors - Yulun Wu
View Article
humanWB

Invest Ophthalmol Vis Sci

Comparative Proteomic Profiling of Microdissected Spindle and Epithelioid Uveal Melanoma Subtypes.

Authors - Yu Luan
View Article
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