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验证数据展示

  • WB analysis of mouse heart using 27931-1-AP

    WB analysis of mouse heart using 27931-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 27931-1-AP (NDUFB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 27931-1-AP

    IHC staining of human stomach cancer using 27931-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27931-1-AP (NDUFB4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 27931-1-AP

    IF Staining of HeLa using 27931-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFB4 antibody (27931-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

NDUFB4 Polyclonal antibody

NDUFB4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27931-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

NADH-ubiquinone oxidoreductase B15 subunit, Complex I-B15, Complex I B15, CI-B15, CI B15

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27931-1-AP targets NDUFB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 NDUFB4 fusion protein Ag27484 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa
别名 NADH-ubiquinone oxidoreductase B15 subunit, Complex I-B15, Complex I B15, CI-B15, CI B15
计算分子量 15 kDa
观测分子量 15 kDa
GenBank蛋白编号BC000855
基因名称 NDUFB4
Gene ID (NCBI) 4710
RRIDAB_2881012
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95168
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NDUFB4 antibody 27931-1-APDownload protocol
IHC protocol for NDUFB4 antibody 27931-1-APDownload protocol
IF protocol for NDUFB4 antibody 27931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols