验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, mouse spleen tissue, human heart tissue, mouse heart tissue, rat heart tissue, HuH-7 cells, MCF-7 cells, mouse brain tissue, mouse kidney tissue |
| Positive IHC detected in | mouse brain tissue, human heart tissue, human kidney tissue, human skin tissue, human liver tissue, human ovary tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | C2C12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
17614-1-AP targets NDUFB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | NDUFB2 fusion protein Ag11789 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa |
| 别名 | NADH-ubiquinone oxidoreductase AGGG subunit, Complex I-AGGG, Complex I AGGG, CI-AGGG, CI AGGG |
| 计算分子量 | 95 aa, 11 kDa |
| 观测分子量 | 8-12 kDa |
| GenBank蛋白编号 | BC063026 |
| 基因名称 | NDUFB2 |
| Gene ID (NCBI) | 4708 |
| RRID | AB_2150944 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95178 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. NDUFB2, also named as CI-AGGG, is a nuclear encoded subunit located in the hydrophobic protein fraction of complex I(PMID:9878551). It may have a role in the regulation of molecular changes during the global cardiac ischemia/reperfusion injury during cardiopulmonary bypass (CPB). The full length 12 kDa protein has a transit peptide with 33 amino acids.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NDUFB2 antibody 17614-1-AP | Download protocol |
| IHC protocol for NDUFB2 antibody 17614-1-AP | Download protocol |
| IF protocol for NDUFB2 antibody 17614-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
