验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 13 publications below |
产品信息
16720-1-AP targets NDUFB11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag10159 Product name: Recombinant human NDUFB11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 30-163 aa of BC107805 Sequence: ESSFSRTVVAPSAVAGKRPPEPTTPWQEDPEPEDENLYEKNPDSHGYDKDPVLDVWNMRLVFFFGVSIILVLGSTFVAYLPDYRCTGCPRAWDGMKEWSRREAERLVKYREANGLPIMESNCFDPSKIQLPEDE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 11, 17.3kDa |
| 别名 | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial, ESSS, Complex I-ESSS, Complex I ESSS, CI-ESSS |
| 计算分子量 | 163 aa, 18 kDa |
| 观测分子量 | 18-20 kDa |
| GenBank蛋白编号 | BC107805 |
| 基因名称 | NDUFB11 |
| Gene ID (NCBI) | 54539 |
| RRID | AB_2298378 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NX14 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NDUFB11(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial) is also named as neuronal protein 17.3(Np17.3) and belongs to the complex I NDUFB11 subunit family. This protein is involved in the transfer of electrons from NADH to the respiratory chain and play a role in the growth, maintenance, and survival of neurons. NDUFB11 has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NDUFB11 antibody 16720-1-AP | Download protocol |
| IHC protocol for NDUFB11 antibody 16720-1-AP | Download protocol |
| IF protocol for NDUFB11 antibody 16720-1-AP | Download protocol |
| IP protocol for NDUFB11 antibody 16720-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria | ||
EMBO J Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV. | ||
Am J Hum Genet Mutations in NDUFB11, Encoding a Complex I Component of the Mitochondrial Respiratory Chain, Cause Microphthalmia with Linear Skin Defects Syndrome.
| ||
iScience Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1 | ||
J Biol Chem Complex I mutations synergize to worsen the phenotypic expression of Leber's hereditary optic neuropathy. | ||
Clin Genet A novel mutation in NDUFB11 unveils a new clinical phenotype associated with lactic acidosis and sideroblastic anemia. |
