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NDUFB1 Polyclonal antibody

NDUFB1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16902-1-AP

Print datasheet

Synonyms

CI MNLL, CI SGDH, Complex I MNLL, MNLL, NDUFB1

验证数据展示

  • WB analysis of A375 using 16902-1-AP

    WB analysis of A375 using 16902-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 16902-1-AP (NDUFB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16902-1-AP

    IHC staining of human kidney using 16902-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16902-1-AP (NDUFB1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 16902-1-AP

    IHC staining of human kidney using 16902-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16902-1-AP (NDUFB1 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inA375 cells, mouse heart tissue, human colon tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

16902-1-AP targets NDUFB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen NDUFB1 fusion protein Ag10443 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 1, 7kDa
Synonyms CI MNLL, CI SGDH, Complex I MNLL, MNLL, NDUFB1
Calculated Molecular Weight12 kDa, 7 kDa
Observed Molecular Weight 7 kDa
GenBank Accession NumberBC009691
Gene Symbol NDUFB1
Gene ID (NCBI) 4707
RRIDAB_2150787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75438
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NDUFB1 antibody 16902-1-APDownload protocol
IHC protocol for NDUFB1 antibody 16902-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Complex I mutations synergize to worsen the phenotypic expression of Leber's hereditary optic neuropathy.

Authors - Yanchun Ji
View Article
WB

Int J Neurosci

Dysferlinopathy: Mitochondrial Abnormalities in Human Skeletal Muscle.

Authors - Fuchen Liu
View Article
humanWB

Cell Rep

A membrane arm of mitochondrial complex I sufficient to promote respirasome formation.

Authors - Hezhi Fang
View Article