验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IP | See 1 publications below |
产品信息
13891-1-AP targets NDUFAF2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag4853 Product name: Recombinant human NDUFAF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-169 aa of BC033965 Sequence: MGWSQDLFRALWRSLSREVKEHVGTDQFGNKYYYIPQYKNWRGQTIREKRIVEAANKKEVDYEAGDIPTEWEAWIRRTRKTPPTMEEILKNEKHREEIKIKSQDFYEKEKLLSKETSEELLPPPVQTQIKGHASAPYFGKEEPSVAPSSTGKTFQPGSWMPRDGKSHNQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2 |
| 别名 | B17.2 like, B17.2L, mimitin, Mimitin, mitochondrial, MMTN, NDUFA12 like protein, NDUFA12L, NDUFAF2 |
| 计算分子量 | 169 aa, 20 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC033965 |
| 基因名称 | NDUFAF2 |
| Gene ID (NCBI) | 91942 |
| RRID | AB_10597105 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N183 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mimitin, also named as MMTN, B17.2L, NDUFAF2 and NDUFA12L, is a small mitochondrial protein upregulated by proinflammatory cytokines at the transcriptional and protein levels. It is reported as a possible chaperone for assembly of mitochondrial complex I. The MW of Mimitin is 17-25 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NDUFAF2 antibody 13891-1-AP | Download protocol |
| IP protocol for NDUFAF2 antibody 13891-1-AP | Download protocol |
| WB protocol for NDUFAF2 antibody 13891-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV. | ||
J Med Genet Respiratory chain complex I deficiency due to NDUFA12 mutations as a new cause of Leigh syndrome. | ||
Redox Rep Ndufaf2, a protein in mitochondrial complex I, interacts in vivo with methionine sulfoxide reductases |
