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验证数据展示

  • WB analysis using 87143-2-RR (same clone as 87143-2-PBS)

    WB analysis using 87143-2-RR (same clone as 87143-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87143-2-RR (NDUFA9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87143-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 87143-2-RR (same clone as 87143-2-PBS)

    IF Staining of HeLa using 87143-2-RR (same clone as 87143-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFA9 antibody (87143-2-RR, Clone: 252374E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87143-2-PBS in a different storage buffer formulation.

  • IHC staining of Human lung cancer （Adenocarcinoma） using 87143-2-RR (same clone as 87143-2-PBS)

    IHC staining of Human lung cancer （Adenocarcinoma） using 87143-2-RR (same clone as 87143-2-PBS)

    Immunohistochemical analysis of paraffin-embedded Human lung cancer (Adenocarcinoma) slide using 87143-2-RR (NDUFA9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87143-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 87143-2-RR (same clone as 87143-2-PBS)

    IHC staining of mouse heart using 87143-2-RR (same clone as 87143-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 87143-2-RR (NDUFA9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87143-2-PBS in a different storage buffer formulation.

NDUFA9 Recombinant monoclonal antibody, PBS Only

NDUFA9 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 87143-2-PBS

产品说明书

CloneNo. 252374E1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CI 39kD, CI-39kD, Complex I-39kD, NADH-ubiquinone oxidoreductase 39 kDa subunit, NDUFS2L

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87143-2-PBS targets NDUFA9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16832

Product name: Recombinant human NDUFA9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-273 aa of BC009311

Sequence: MAAAAQSRVVRVLSMSRSAITAIATSVCHGPPCRQLHHALMPHGKGGRSSVSGIVATVFGATGFLGRYVVNHLGRMGSQVIIPYRCDKYDIMHLRPMGDLGQLLFLEWDARDKDSIRRVVQHSNVVINLIGRDWETKNFDFEDVFVKIPQAIAQLSKEAGVEKFIHVSHLNANIKSSSRYLRNKAVGEKVVRDAFPEAIIVKPSDIFGREDRFLNSFASMHRFGPIPLGSLGWKTVKQPVYVVDVSKGIVNAVKDPDANGKSFAFVGPSRYLL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa
别名 CI 39kD, CI-39kD, Complex I-39kD, NADH-ubiquinone oxidoreductase 39 kDa subunit, NDUFS2L
计算分子量 377 aa, 43 kDa
观测分子量35 kDa
GenBank蛋白编号BC009311
基因名称 NDUFA9
Gene ID (NCBI) 4704
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16795
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDUFA9 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial) is also named as NDUFS2L, CI-39kD and belongs to the complex I NDUFA9 subunit family. It is involved in stabilizing the junction between membrane and matrix arms of complex I, a late assembly step critical for complex I biogenesis and activity.

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