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验证数据展示

  • WB analysis using 29621-1-AP

    WB analysis using 29621-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29621-1-AP (NDUFA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 29621-1-AP

    IHC staining of human hepatocirrhosis using 29621-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 29621-1-AP (NDUFA9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human hepatocirrhosis using 29621-1-AP

    IHC staining of human hepatocirrhosis using 29621-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 29621-1-AP (NDUFA9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NDUFA9 Polyclonal antibody

NDUFA9 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29621-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Rat

应用

WB, IHC, ELISA

别名

CI 39kD, Complex I 39kD, NDUFA9, NDUFS2L, SDR22E1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, U2OS cells, mouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29621-1-AP targets NDUFA9 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag31222

Product name: Recombinant human NDUFA9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 108-227 aa of BC009311

Sequence: WDARDKDSIRRVVQHSNVVINLIGRDWETKNFDFEDVFVKIPQAIAQLSKEAGVEKFIHVSHLNANIKSSSRYLRNKAVGEKVVRDAFPEAIIVKPSDIFGREDRFLNSFASMHRFGPIP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa
别名 CI 39kD, Complex I 39kD, NDUFA9, NDUFS2L, SDR22E1
计算分子量 377 aa, 43 kDa
观测分子量35 kDa
GenBank蛋白编号BC009311
基因名称 NDUFA9
Gene ID (NCBI) 4704
RRIDAB_2918331
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16795
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDUFA9 (NADH: ubiquinone oxidoreductase subunit A9), also named as NDUFS2L, belongs to the complex I NDUFA9 subunit family. NDUFA9 gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Instead of being implicated in the catalysis, NDUFA9 is required for proper assembly of the complex I (PMID: 34938809). The predicted molecular weight of NDUFA9 is 42 kDa. With modification, the MW of NDUFA9 will be migrated to 35 kDa.

实验方案

Product Specific Protocols
IHC protocol for NDUFA9 antibody 29621-1-APDownload protocol
WB protocol for NDUFA9 antibody 29621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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