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验证数据展示

  • WB analysis using 84498-1-RR (same clone as 84498-1-PBS)

    WB analysis using 84498-1-RR (same clone as 84498-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84498-1-RR (NDUFA4L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84498-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 84498-1-RR (same clone as 84498-1-PBS)

    IHC staining of mouse kidney using 84498-1-RR (same clone as 84498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84498-1-RR (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84498-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFA4L2 antibody included
  • IHC staining of mouse kidney using 84498-1-RR (same clone as 84498-1-PBS)

    IHC staining of mouse kidney using 84498-1-RR (same clone as 84498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84498-1-RR (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84498-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFA4L2 antibody included
  • IF Staining of HeLa using 84498-1-RR (same clone as 84498-1-PBS)

    IF Staining of HeLa using 84498-1-RR (same clone as 84498-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFA4L2 antibody (84498-1-RR, Clone: 241622B12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84498-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84498-1-RR

    Affinity and Kinetic Characterization of 84498-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84498-1-RR against Human NDUFA4L2 were performed. The affinity constant is below 1 pM.

NDUFA4L2 Recombinant antibody, PBS Only

NDUFA4L2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84498-1-PBS

产品说明书

CloneNo. 241622B12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, 241622B12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NDUFA4L2 Ready-To-Use IHC Kit. NDUFA4L2 primary antibody included.

产品信息

84498-1-PBS targets NDUFA4L2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 NDUFA4L2 fusion protein Ag9600 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
别名 NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, 241622B12
计算分子量 87 aa, 10 kDa
观测分子量 10 kDa
GenBank蛋白编号BC011910
基因名称 NDUFA4L2
Gene ID (NCBI) 56901
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NRX3
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDUFA4L2 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2), also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria and belongs to the complex I NDUFA4 subunit family. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.