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  • Featured Product
  • KD/KO Validated

DAP13/NDUFA12 Polyclonal antibody

DAP13/NDUFA12 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15793-1-AP

Print datasheet

Synonyms

B17.2, CI B17.2, CIB17.2, Complex I B17.2, DAP13, NDUFA12

验证数据展示

  • WB analysis using 15793-1-AP

    WB analysis using 15793-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15793-1-AP (DAP13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15793-1-AP

    IF Staining of HepG2 using 15793-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15793-1-AP (DAP13 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse skeletal muscle using 15793-1-AP

    IHC staining of mouse skeletal muscle using 15793-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15793-1-AP (DAP13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 15793-1-AP

    IHC staining of mouse skeletal muscle using 15793-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15793-1-AP (DAP13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, mouse kidney tissue, mouse heart tissue, rat kidney tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15793-1-AP targets DAP13/NDUFA12 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen DAP13/NDUFA12 fusion protein Ag8501 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12
Synonyms B17.2, CI B17.2, CIB17.2, Complex I B17.2, DAP13, NDUFA12
Calculated Molecular Weight 17 kDa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC005936
Gene Symbol NDUFA12
Gene ID (NCBI) 55967
RRIDAB_2150475
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UI09
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DAP13/NDUFA12 antibody 15793-1-APDownload protocol
IHC protocol for DAP13/NDUFA12 antibody 15793-1-APDownload protocol
IF protocol for DAP13/NDUFA12 antibody 15793-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Theranostics

Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I.

Authors - Lujing Jiang
View Article
humanWB

Hum Mutat

Novel NDUFA12 variants are associated with isolated complex I defect and variable clinical manifestation.

Authors - Alessandra Torraco
View Article