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验证数据展示

  • WB analysis using 32947-1-AP (same clone as 32947-1-PBS)

    WB analysis using 32947-1-AP (same clone as 32947-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32947-1-AP (NDUFA10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.

  • WB analysis using 32947-1-AP (same clone as 32947-1-PBS)

    WB analysis using 32947-1-AP (same clone as 32947-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32947-1-AP (NDUFA10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 32947-1-AP (same clone as 32947-1-PBS)

    IHC staining of human ovary tumor using 32947-1-AP (same clone as 32947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 32947-1-AP (NDUFA10 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFA10 antibody included
  • IF Staining of A431 using 32947-1-AP (same clone as 32947-1-PBS)

    IF Staining of A431 using 32947-1-AP (same clone as 32947-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NDUFA10 antibody (32947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 32947-1-AP (same clone as 32947-1-PBS)

    IF Staining of HeLa using 32947-1-AP (same clone as 32947-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFA10 antibody (32947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.

NDUFA10 Polyclonal antibody, PBS Only

NDUFA10 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 32947-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CI-42kD, Complex I-42kD, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial, NADH:Ubiquinone Oxidoreductase Subunit A10, NADH-ubiquinone oxidoreductase 42 kDa subunit

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® NDUFA10 Ready-To-Use IHC Kit. NDUFA10 primary antibody included.

产品信息

32947-1-PBS targets NDUFA10 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag37576

Product name: Recombinant human NDUFA10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-355 aa of BC003417

Sequence: MALRLLKLAATSASARVVAAGAQRVRGIHSSVQCKLRYGMWHFLLGDKASKRLTERSRVITVDGNICTGKGKLAKEIAEKLGFKHFPEAGIHYPDSTTGDGKPLATDYNGNCSLEKFYDDPRSNDGNSYRLQSWLYSSRLLQYSDALEHLLTTGQGVVLERSIFSDFVFLEAMYNQGFIRKQCVDHYNEVKSVTICDYLPPHLVIYIDVPVPEVQRRIQKKGDPHEMKITSAYLQDIENAYKKTFLPEMSEKCEVLQYSAREAQDSKKVVEDIEYLKFDKGPWLKQDNRTLYHLRLLVQDKFEVLNYTSIPIFLPEVTIGAHQTDRVLHQFRELPGRKYSPGYNTEVGDKWIWLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa
别名 CI-42kD, Complex I-42kD, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial, NADH:Ubiquinone Oxidoreductase Subunit A10, NADH-ubiquinone oxidoreductase 42 kDa subunit
计算分子量 41 kDa
观测分子量40 kDa
GenBank蛋白编号BC003417
基因名称 NDUFA10
Gene ID (NCBI) 4705
RRIDAB_3742801
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO95299
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDUFA10 (NADH:ubiquinone oxidoreductase subunit A10) is a subunit of mitochondrial complex I of the electron transport chain, encoding a protein with NADH dehydrogenase and oxidoreductase activity. This protein transfers electrons from NADH to the respiratory chain. Mutations in the NDUFA10 gene are associated with Leigh syndrome, a common mitochondrial energy metabolism disorder in children. Additionally, the expression levels of NDUFA10 are related to the occurrence and prognosis of various diseases. For example, in Alzheimer's disease, its low expression is associated with increased dementia severity. In prostate cancer, low expression of NDUFA10 predicts a poor prognosis.

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