验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21569-1-AP targets NDST3 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag16091 Product name: Recombinant human NDST3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 180-261 aa of BC109309 Sequence: FPFSIYGNLAVKDCCINPHSPLIRVTKSSKLEKGSLPGTDWTVFQINHSAYQPVIFAKVKTPENLSPSISKGAFYATIIHDL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 |
| 别名 | hNDST-3, HSST3, NDST-3 |
| 计算分子量 | 873 aa, 101 kDa |
| 观测分子量 | 44 kDa, 64 kDa, 101 kDa |
| GenBank蛋白编号 | BC109309 |
| 基因名称 | NDST3 |
| Gene ID (NCBI) | 9348 |
| RRID | AB_3742044 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95803 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NDST3 (Glucosaminyl N-deacetylase/N-sulfotransferase 3) is known to catalyze deacetylation and N-sulfation on disaccharide substrates. Aberrant NDST3 expression or tubulin acetylation has been observed in an increasing number of NDs, including amyotrophic lateral sclerosis and frontotemporal dementia (ALS/FTD), schizophrenia and bipolar disorder, Alzheimer's disease (AD), and Parkinson's disease (PD) (PMID: 41268696, 34435379).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NDST3 antibody 21569-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
