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验证数据展示

  • WB analysis using 31421-1-AP

    WB analysis using 31421-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31421-1-AP (NDRG3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 31421-1-AP

    WB analysis of A549 using 31421-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31421-1-AP (NDRG3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 31421-1-AP

    IHC staining of human colon using 31421-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 31421-1-AP (NDRG3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NDRG3 Polyclonal antibody

NDRG3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31421-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Protein NDRG3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, Raji cells, Calu-1 cells, Ramos cells, SKOV-3 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31421-1-AP targets NDRG3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33626

Product name: Recombinant human NDRG3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 178-237 aa of NM_032013

Sequence: LSGLTTNVVDIILAHHFGQEELQANLDLIQTYRMHIAQDINQDNLQLFLNSYNGRRDLEI*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NDRG family member 3
别名 Protein NDRG3
计算分子量41 kDa
观测分子量41-45 kDa
GenBank蛋白编号NM_032013
基因名称 NDRG3
Gene ID (NCBI) 57446
RRIDAB_3669974
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9UGV2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDRG (N-Myc downstream-regulated gene) family is generally divided into two subfamilies, one composed of NDRG1 and NDRG3 with a homology of 67%, and the other composed of NDRG2 and NDRG4 with a homology of 58%. NDRG3 is a downstream regulatory factor of MYC, with a total length of 2588 base pairs. It is mainly expressed in ovary, prostate, testis, brain, spinal cord, thymus, heart and kidney. NDRG3 is located on chromosome 20q11.21-11.23 and encodes at least two subtypes, 375 and 363 amino acids, respectively, with an apparent molecular weight of 41 and 40 kDa, respectively. (PMID: 36619553)

实验方案

Product Specific Protocols
IHC protocol for NDRG3 antibody 31421-1-APDownload protocol
WB protocol for NDRG3 antibody 31421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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