验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse kidney tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26902-1-AP targets NDRG1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag25424 Product name: Recombinant human NDRG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 45-109 aa of BC006260 Sequence: SGSSVTSLDGTRSRSHTSEGTRSRSHTSEGTRSRSHTSEGAHLDITPNSGAAGNSAGPKSMEVSC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | N-myc downstream regulated 1 |
| 别名 | CMT4D, Differentiation-related gene 1 protein, DRG1, DRG-1, Protein NDRG1 |
| 计算分子量 | 43 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | BC006260 |
| 基因名称 | NDRG1 |
| Gene ID (NCBI) | 10397 |
| RRID | AB_2880676 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92597 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NDRG1 is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. NDRG1 is a highly conserved cytoplasmic protein involved in cell growth arrest and proliferation, cell differentiation, DNA damage response, heavy metal response, the hypoxia response, tumorigenesis, and metastasis. NDRG1 is a potent metastasis suppressor in multiple tumor-types. NDRG1 appears to serve as a putative tumor suppressor and is down-regulated in colorectal, gastric, cervical, ovarian and prostate cancers.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NDRG1 antibody 26902-1-AP | Download protocol |
| IHC protocol for NDRG1 antibody 26902-1-AP | Download protocol |
| WB protocol for NDRG1 antibody 26902-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Aging Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer | ||
Mol Cell mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis. | ||
Cell Oncol (Dordr) LINC01419 promotes cell proliferation and metastasis in hepatocellular carcinoma by enhancing NDRG1 promoter activity. | ||
Gynecol Oncol Deferasirox shows inhibition activity against cervical cancer in vitro and in vivo. | ||
Mol Biol Rep Excessive iodine induces thyroid follicular epithelial cells apoptosis by activating HIF-1α-mediated hypoxia pathway in Hashimoto thyroiditis |
