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验证数据展示

  • WB analysis using 15602-1-AP (same clone as 15602-1-PBS)

    WB analysis using 15602-1-AP (same clone as 15602-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15602-1-AP (NDFIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15602-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 15602-1-AP (same clone as 15602-1-PBS)

    IP experiment of HEK-293 using 15602-1-AP (same clone as 15602-1-PBS)

    IP result of anti-NDFIP1 (IP:15602-1-AP, 3ug; Detection:15602-1-AP 1:300) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 15602-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 15602-1-AP (same clone as 15602-1-PBS)

    IF Staining of HEK-293 using 15602-1-AP (same clone as 15602-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using NDFIP1 antibody (15602-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15602-1-PBS in a different storage buffer formulation.

NDFIP1 Polyclonal antibody, PBS Only

NDFIP1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 15602-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15602-1-PBS targets NDFIP1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7985

Product name: Recombinant human NDFIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-119 aa of BC004317

Sequence: MALALAALAAVEPACGSRYQQLQNEEESGEPEQAAGDAPPPYSSISAESAAYFDYKDESGFPKPPSYNVATTLPSYDEAERTKAEATIPLVPGRDEDFVGRDDFDDADQLRIGNDGIFM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Nedd4 family interacting protein 1
别名
计算分子量 25 kDa
观测分子量 25-30 kDa
GenBank蛋白编号BC004317
基因名称 NDFIP1
Gene ID (NCBI) 80762
RRIDAB_2878157
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BT67
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ndfip1 is an adaptor protein for the Nedd4 family of ubiquitin ligases and has been found to be upregulated in neurons after brain injury, including head trauma, stroke and metal toxicity. Recent studies confirmed the role of Ndfip1 as a regulator of iron metabolism and pointed out that Ndfip1 was involved in iron homeostasis by regulating the degradation of iron importer divalent metal transporter 1. Ndfip1 is a transmembrane protein that is localized to the Golgi and post-Golgi vesicles such as endosomes. It is an adaptor protein that recruits E3 ligases to ubiquitinate target proteins. It is ubiquitously expressed and contains 2 PY motifs, which interact with several Nedd4 family E3s to degradate proteins.

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