验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, SGC-7901 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy® ND4 Ready-To-Use IHC Kit. ND4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85098-1-RR targets ND4 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | NADH dehydrogenase, subunit 4 (complex I) |
| 别名 | MT-ND4, 242422E2, MT ND4, MTND4, NADH4 |
| 计算分子量 | 52 kDa |
| 观测分子量 | 43-52 kDa |
| GenBank蛋白编号 | YP_003024035 |
| 基因名称 | ND4 |
| Gene ID (NCBI) | 4538 |
| RRID | AB_3744010 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P03905 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NADH dehydrogenase 4 (ND4) is one of the core subunits of mitochondrial respiratory chain Complex I and is essential for cellular energy production. Complex I is the first step involved in cellular aerobic respiration (i.e., oxidative phosphorylation), responsible for catalyzing the transfer of electrons from NADH to coenzyme Q10 (ubiquinone), thereby initiating the entire electron transport chain.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ND4 antibody 85098-1-RR | Download protocol |
| WB protocol for ND4 antibody 85098-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
