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SRC-3/AIB1 Polyclonal antibody

SRC-3/AIB1 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, WB,ELISA

Conjugate

Unconjugated

Cat No : 22707-1-AP

Print datasheet

Synonyms

ACTR, AIB 1, AIB1, KAT13B, NCoA 3, NCOA3, nuclear receptor coactivator 3, pCIP, RAC 3, RAC3, SRC 3, SRC3, TRAM 1, TRAM1

验证数据展示

  • WB analysis of Raji using 22707-1-AP

    WB analysis of Raji using 22707-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 22707-1-AP (SRC-3/AIB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of Raji using 22707-1-AP

    FC experiment of Raji using 22707-1-AP

    1X10^6 Raji cells were stained with 0.2ug SRC-3/AIB1 antibody (22707-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

经过测试的应用

Positive WB detected inRaji cells
Positive FC detected inRaji cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22707-1-AP is SRC-3/AIB1 Fusion Protein expressed in E. coli.

Tested Applications FC, WB,ELISA
Tested Reactivity human
Immunogen SRC-3/AIB1 fusion protein Ag18424 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear receptor coactivator 3
Synonyms ACTR, AIB 1, AIB1, KAT13B, NCoA 3, NCOA3, nuclear receptor coactivator 3, pCIP, RAC 3, RAC3, SRC 3, SRC3, TRAM 1, TRAM1
Calculated Molecular Weight 1424 aa, 155 kDa
Observed Molecular Weight 155 kDa
GenBank Accession NumberBC119001
Gene Symbol NCOA3
Gene ID (NCBI) 8202
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6Q9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.