验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, MCF-7 cells, PC-3 cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
51114-1-AP targets NCOA1/SRC-1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear receptor coactivator 1 |
| 别名 | bHLHe42, bHLHe74, F SRC 1, KAT13A, NCoA 1, NCOA1, nuclear receptor coactivator 1, Protein Hin 2, RIP160, SRC 1, SRC1, Steroid receptor coactivator 1 |
| 计算分子量 | 1441 aa, 157 kDa |
| 观测分子量 | 140-160 kDa |
| GenBank蛋白编号 | BC111533 |
| 基因名称 | SRC 1/NCOA1 |
| Gene ID (NCBI) | 8648 |
| RRID | AB_2150905 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15788 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NCOA1/SRC-1 antibody 51114-1-AP | Download protocol |
| WB protocol for NCOA1/SRC-1 antibody 51114-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
