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验证数据展示

  • WB analysis of mouse testis using 51114-1-AP

    WB analysis of mouse testis using 51114-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 51114-1-AP (NCOA1/SRC-1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 51114-1-AP

    IHC staining of human testis using 51114-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 51114-1-AP (NCOA1/SRC-1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 51114-1-AP

    IHC staining of human testis using 51114-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 51114-1-AP (NCOA1/SRC-1 antibody) at dilution of 1:100 (under 40x lens).

NCOA1/SRC-1 Polyclonal antibody

NCOA1/SRC-1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 51114-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

bHLHe42, bHLHe74, F SRC 1, KAT13A, NCoA 1, NCOA1, nuclear receptor coactivator 1, Protein Hin 2, RIP160, SRC 1, SRC1, Steroid receptor coactivator 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51114-1-AP targets NCOA1/SRC-1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear receptor coactivator 1
别名 bHLHe42, bHLHe74, F SRC 1, KAT13A, NCoA 1, NCOA1, nuclear receptor coactivator 1, Protein Hin 2, RIP160, SRC 1, SRC1, Steroid receptor coactivator 1
计算分子量 1441 aa, 157 kDa
观测分子量 140-160 kDa
GenBank蛋白编号BC111533
基因名称 SRC 1/NCOA1
Gene ID (NCBI) 8648
RRIDAB_2150905
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15788
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NCOA1/SRC-1 antibody 51114-1-APDownload protocol
IHC protocol for NCOA1/SRC-1 antibody 51114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols