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验证数据展示

  • WB analysis of mouse pancreas using 10985-1-AP

    WB analysis of mouse pancreas using 10985-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10985-1-AP (NCLN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 10985-1-AP

    IHC staining of human stomach cancer using 10985-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10985-1-AP (NCLN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 10985-1-AP

    IHC staining of human stomach cancer using 10985-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10985-1-AP (NCLN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NCLN Polyclonal antibody

NCLN Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10985-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

NCLN, Nicalin, nicalin homolog (zebrafish), Nicastrin like protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman stomach cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10985-1-AP targets NCLN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NCLN fusion protein Ag1430 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nicalin homolog (zebrafish)
别名 NCLN, Nicalin, nicalin homolog (zebrafish), Nicastrin like protein
计算分子量 60 kDa
观测分子量 60-64 kDa
GenBank蛋白编号BC013283
基因名称 NCLN
Gene ID (NCBI) 56926
RRIDAB_2150898
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969V3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nicalin, also named as NCLN, is a key component of a protein complex that antagonizes Nodal signaling, which in vertebrates is involved in induction of the mesoderm and endoderm. Nicalin is highest expression in pancreas and skeletal muscle, with somewhat lower expression in heart and very low expression in all other tissues examined. Endogenous nicalin has an apparent molecular mass of 60 kDa. Nicalin was also detected in a 500- to 550-kDa complex containing NOMO2.

实验方案

Product Specific Protocols
WB protocol for NCLN antibody 10985-1-APDownload protocol
IHC protocol for NCLN antibody 10985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols