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CoraLite® Plus 647-conjugated NCL Polyclonal antibody

NCL Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-10556

Print datasheet

Synonyms

C23, FLJ45706, NCL, nucleolin, Protein C23

验证数据展示

  • FC experiment of HeLa using CL647-10556

    FC experiment of HeLa using CL647-10556

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human NCL (CL647-10556) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NCL Ready-To-Use IHC Kit. NCL primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10556-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10556 targets NCL in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse, rat
Immunogen NCL fusion protein Ag0859 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nucleolin
Synonyms C23, FLJ45706, NCL, nucleolin, Protein C23
Calculated Molecular Weight 76 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC006494
Gene Symbol NCL
Gene ID (NCBI) 4691
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19338
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation. Whilst mammalian nucleolin has a predicted molecular mass of approximately 77 kDa, the apparent molecular mass is between 100 and 110 kDa, and has been attributed to the amino acid composition of the N-terminal domain, which is highly phosphorylated. (PMID: 15925566)

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 NCL antibody CL647-10556Download protocol
Standard Protocols
Click here to view our Standard Protocols