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验证数据展示

  • WB analysis using 10556-1-AP (same clone as 10556-1-PBS)

    WB analysis using 10556-1-AP (same clone as 10556-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10556-1-AP (Nucleolin/C23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10556-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 10556-1-PBS

    IF Staining of HEK-293 using 10556-1-PBS

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NCL antibody (10556-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IP experiment of HeLa using 10556-1-AP (same clone as 10556-1-PBS)

    IP experiment of HeLa using 10556-1-AP (same clone as 10556-1-PBS)

    IP result of anti-Nucleolin/C23 (IP:10556-1-AP, 4ug; Detection:10556-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 10556-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 10556-1-AP (same clone as 10556-1-PBS)

    IHC staining of human lung cancer using 10556-1-AP (same clone as 10556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10556-1-AP (Nucleolin/C23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10556-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NCL antibody included
  • IF Staining of HEK-293 using 10556-1-AP (same clone as 10556-1-PBS)

    IF Staining of HEK-293 using 10556-1-AP (same clone as 10556-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NCL antibody (10556-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 10556-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Nucleolin/C23 Polyclonal antibody, PBS Only

Nucleolin/C23 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10556-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

C23, nucleolin, FLJ45706, NCL, Protein C23

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NCL Ready-To-Use IHC Kit. NCL primary antibody included.

产品信息

10556-1-PBS targets Nucleolin/C23 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0859

Product name: Recombinant human NCL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-482 aa of BC006494

Sequence: MVKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGEEVVIPQKKGKKAAATSAKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPAKAVTTPGKKGATPGKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDDEDEDEDEDEIEPAAMKAAAAAPASEDEDDEDDEDDEDGKSKGIAYIEFKTEADAEKTFEEKQGTEIDGRSISLYYTGEKGQNQDYRGGKNSTWSGESKTLVLSNLSYSATEETLQEVFEKATFIKVPQNQNGKSKGYAFIEFASFEDAKEALNSCNKREIEGRAIRLELQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFDGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleolin
别名 C23, nucleolin, FLJ45706, NCL, Protein C23
计算分子量 76 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC006494
基因名称 NCL
Gene ID (NCBI) 4691
RRIDAB_2082423
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19338
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation. Whilst mammalian nucleolin has a predicted molecular mass of approximately 77 kDa, the apparent molecular mass is between 100 and 110 kDa, and has been attributed to the amino acid composition of the N-terminal domain, which is highly phosphorylated. (PMID: 15925566)

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