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验证数据展示

  • WB analysis using 31199-1-AP (same clone as 31199-1-PBS)

    WB analysis using 31199-1-AP (same clone as 31199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31199-1-AP (NCKAP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31199-1-PBS in a different storage buffer formulation.

  • WB analysis using 31199-1-AP (same clone as 31199-1-PBS)

    WB analysis using 31199-1-AP (same clone as 31199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31199-1-AP (NCKAP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31199-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 31199-1-AP (same clone as 31199-1-PBS)

    IF Staining of U2OS using 31199-1-AP (same clone as 31199-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NCKAP5 antibody (31199-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31199-1-PBS in a different storage buffer formulation.

NCKAP5 Polyclonal antibody, PBS Only

NCKAP5 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 31199-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

NAP5, Peripheral clock protein, Nck associated protein 5, NAP 5, ERIH2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31199-1-PBS targets NCKAP5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag35092

Product name: Recombinant human NCKAP5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-115 aa of NM_207363

Sequence: MEGKRQLEKRDFGKRLSLDSSLVEYMDSNKYIEHLLTQLEEQHRSLWREKLAVARLQREVAQRTSEGAMHEKLIHELEEERHLRLQSEKRLQEVTLESERNRIQMRSLQQQFSRM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Nck-associated protein 5
别名 NAP5, Peripheral clock protein, Nck associated protein 5, NAP 5, ERIH2
计算分子量209 kDa
观测分子量250-260 kDa
GenBank蛋白编号NM_207363
基因名称 NAP5
Gene ID (NCBI) 344148
RRIDAB_3669897
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO14513
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NCKAP5 (Nck-associated protein 5), also known as NAP5. NCKAP5 is predicted to be located in cells, especially in nucleoli, Golgi apparatus, cell membrane and cytoplasm. It is widely expressed in many tissues, especially in lung and kidney, and in more than 20 other tissues. And the expression of NCKAP5 was enhanced in oligodendrocytes, oligodendrocyte precursor cells and astrocytes. It may interact with other protein, such as NPM1 and centromere protein complex, and regulate accurate chromosome segregation and distribution. NCKAP5 may be involved in the formation and development of tumors, especially in the process of tumor variation such as ovary and breast cancer. In addition, NCKAP5 may be a diagnostic biomarker in non-small cell lung cancer (NSCLC), which is related to the infiltration of activated B cells, regulatory T cells (Tregs), neutrophils and dendritic cells (PMID: 36628881). The molecular weight of NCKAP5 is 208 kDa, it also has some protein modifications.

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