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验证数据展示

  • WB analysis using 12140-1-AP (same clone as 12140-1-PBS)

    WB analysis using 12140-1-AP (same clone as 12140-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12140-1-AP (NCKAP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 12140-1-AP (same clone as 12140-1-PBS)

    IF Staining of mouse brain using 12140-1-AP (same clone as 12140-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCKAP1 antibody (12140-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 12140-1-AP (same clone as 12140-1-PBS)

    IHC staining of mouse heart using 12140-1-AP (same clone as 12140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12140-1-AP (NCKAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NCKAP1 antibody included
  • IHC staining of mouse brain using 12140-1-AP (same clone as 12140-1-PBS)

    IHC staining of mouse brain using 12140-1-AP (same clone as 12140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12140-1-AP (NCKAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NCKAP1 antibody included
  • IHC staining of mouse brain using 12140-1-AP (same clone as 12140-1-PBS)

    IHC staining of mouse brain using 12140-1-AP (same clone as 12140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12140-1-AP (NCKAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NCKAP1 antibody included
  • IF Staining of U-251 using 12140-1-AP (same clone as 12140-1-PBS)

    IF Staining of U-251 using 12140-1-AP (same clone as 12140-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using NCKAP1 antibody (12140-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

NCKAP1 Polyclonal antibody, PBS Only

NCKAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 12140-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

HEM2, KIAA0587, Membrane-associated protein HEM-2, NAP 1, NAP1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® NCKAP1 Ready-To-Use IHC Kit. NCKAP1 primary antibody included.

产品信息

12140-1-PBS targets NCKAP1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2788

Product name: Recombinant human NAP1;NCKAP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 779-1129 aa of BC015025

Sequence: HGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NCK-associated protein 1
别名 HEM2, KIAA0587, Membrane-associated protein HEM-2, NAP 1, NAP1
计算分子量 129 kDa
观测分子量 115-125 kDa
GenBank蛋白编号BC015025
基因名称 NCKAP1
Gene ID (NCBI) 10787
RRIDAB_10666284
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2A7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NCK-associated protein 1 (NCKAP1), also known as NAP1 or HEM2, is encoded by an apoptosis-related gene human NAP1, the expression of which is strongly down-regulated in sporadic Alzheimer's disease (AD). Human NAP1 is predominantly expressed in neuronal cells. Antisense oligo DNA of human NAP1 transcripts was found to induce apoptosis of neuronal cells. Loss of NAP1 function disrupts neuronal differentiation. Likewise, NAP1 plays an essential role in facilitating neuronal cytoskeletal changes underlying the postmigratory differentiation of cortical neurons, a critical step in functional wiring of the cortex. Human NAP1 is also proved to be an orthologue of rat Nap1 which binds to the adaptor molecule NCK in signal transduction.

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