验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, L02 cells, pig liver tissue |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67594-1-Ig targets NOXA2/p67phox in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | Human, pig |
免疫原 | NOXA2/p67phox fusion protein Ag8691 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | neutrophil cytosolic factor 2 |
别名 | FLJ93058, NADPH oxidase activator 2, NCF 2, NCF2, Neutrophil cytosol factor 2, neutrophil cytosolic factor 2, NOXA2, P67 PHOX, P67PHOX |
计算分子量 | 60 kDa |
观测分子量 | 67 kDa |
GenBank蛋白编号 | BC001606 |
基因名称 | NCF2 |
Gene ID (NCBI) | 4688 |
RRID | AB_2882801 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P19878 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for NOXA2/p67phox antibody 67594-1-Ig | Download protocol |
IHC protocol for NOXA2/p67phox antibody 67594-1-Ig | Download protocol |
IF protocol for NOXA2/p67phox antibody 67594-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |