p47 phox Polyclonal antibody
p47 phox Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse and More (2)
Applications
IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | U-937 cells, Raji cells |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
28187-1-AP targets p47 phox in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human, rat |
| Immunogen | p47 phox fusion protein Ag28089 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | neutrophil cytosolic factor 1 |
| Synonyms | FLJ79451, NCF 1, NCF 47K, NCF1, NCF1A, Neutrophil cytosol factor 1, neutrophil cytosolic factor 1, Nox organizer 2, Nox organizing protein 2, NOXO2, p47 phox, p47phox, p47-phox, SH3PXD1A |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC002816 |
| Gene Symbol | NCF1 |
| Gene ID (NCBI) | 653361 |
| RRID | AB_2881084 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14598 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for p47 phox antibody 28187-1-AP | Download protocol |
| IHC protocol for p47 phox antibody 28187-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Hazard Mater Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells. | ||
Oxid Med Cell Longev Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates. | ||
Drug Des Devel Ther Artemisinin Improves Acetylcholine-Induced Vasodilatation in Rats with Primary Hypertension |