验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, PC-12 cells, J774A.1 cells, RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33595-1-AP targets NCAPH2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag38706 Product name: Recombinant mouse Ncaph2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 450-607 aa of NM_001115132 Sequence: VMPEEAADLDAEAMPESLRYEELVRRNVELFIATSQKFIQETELSQRIRDWEDTIQPLLQEQEQHVPFDIHIYGDQLASRFPQLNEWCPFSELVAGQPAFEVCRSMLASLQLANDYTVEITQQPGLEAAVDTMSLRLLTHQRAHTRFQTYAAPSMAQP* 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | non-SMC condensin II complex, subunit H2 |
| 别名 | D15Ertd785e, Kleisin-beta |
| 计算分子量 | 69 kDa |
| 观测分子量 | 68-80 kDa |
| GenBank蛋白编号 | NM_001115132 |
| 基因名称 | Ncaph2 |
| Gene ID (NCBI) | 52683 |
| RRID | AB_3743023 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8BSP2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NCAPH2 is a subunit of the condensin-2 complex involved in chromosome condensation during mitosis and meiosis. Peripheral blood DNA methylation levels in the NCAPH2/LMF2 promoter region were significantly decreased in patients with AD and those with amnestic mild cognitive impairment (aMCI); therefore, these are considered to be a potentially useful biomarker for the diagnosis of AD. (PMID: 33603659)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NCAPH2 antibody 33595-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
