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  • Featured Product
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NCAPH Monoclonal antibody

NCAPH Monoclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D2F11

Cat No : 67655-1-Ig

Print datasheet

Synonyms

Barren homolog protein 1, BRRN, BRRN1, CAP H, CAPH, Condensin complex subunit 2, HCAP H, KIAA0074, NCAPH, XCAP H homolog

验证数据展示

  • WB analysis using 67655-1-Ig

    WB analysis using 67655-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67655-1-Ig (NCAPH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of human liver cancer using 67655-1-Ig

    IHC staining of human liver cancer using 67655-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67655-1-Ig (NCAPH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human liver cancer using 67655-1-Ig

    IF Staining of human liver cancer using 67655-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using NCAPH antibody (67655-1-Ig, Clone: 3D2F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of HEK-293 using 67655-1-Ig

    WB analysis of HEK-293 using 67655-1-Ig

    WB result of NCAPH antibody (67655-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAPH transfected HEK-293 cells.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, NIH/3T3 cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67655-1-Ig targets NCAPH in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF-P, IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen NCAPH fusion protein Ag27748 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name non-SMC condensin I complex, subunit H
Synonyms Barren homolog protein 1, BRRN, BRRN1, CAP H, CAPH, Condensin complex subunit 2, HCAP H, KIAA0074, NCAPH, XCAP H homolog
Calculated Molecular Weight 741 aa, 83 kDa
Observed Molecular Weight 83-100 kDa
GenBank Accession NumberBC024211
Gene Symbol NCAPH
Gene ID (NCBI) 23397
RRIDAB_2918495
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15003
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Non-SMC condensin I complex subunit H (NCAPH) is one of the three non-SMC subunits in condensin I, which belongs to a recently defined superfamily of proteins termed kleisins. Another two non-SMC subunits, CAP-D2 and CAP-G, share a highly degenerate repeating motif known as HEAT repeat. Some studies show that each subunit is essential for viability and plays an important role in mitotic chromosome architecture and segregation. In recent years, researchers found that the high expression of NCAPH was associated with poor prognosis in patients with non-small cell lung cancer and prostate cancer. Downregulation of NCAPH inhibited the proliferation, migration, and invasion of several cancer cells significantly. Moreover, NCAPH was involved in the regulation of mature chromosome condensation and DNA damage. These data suggest that NCAPH may be a key carcinogen involved in the development and progression of human malignant tumors. ( PMID: 28300828, PMID: 33311486 )

实验方案

Product Specific Protocols
WB protocol for NCAPH antibody 67655-1-IgDownload protocol
IHC protocol for NCAPH antibody 67655-1-IgDownload protocol
IF protocol for NCAPH antibody 67655-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols