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验证数据展示

  • WB analysis using 87645-1-RR (same clone as 87645-1-PBS)

    WB analysis using 87645-1-RR (same clone as 87645-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87645-1-RR (NCAPG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87645-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 87645-1-RR (same clone as 87645-1-PBS)

    IF Staining of HepG2 using 87645-1-RR (same clone as 87645-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NCAPG2 antibody (87645-1-RR, Clone: 253099H6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87645-1-PBS in a different storage buffer formulation.

NCAPG2 Recombinant monoclonal antibody, PBS Only

NCAPG2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87645-1-PBS

产品说明书

CloneNo. 253099H6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

CAP-G2, Chromosome-associated protein G2, Condensin-2 complex subunit G2, hCAP-G2, Leucine zipper protein 5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87645-1-PBS targets NCAPG2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34991

Product name: Recombinant human NCAPG2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 928-1143 aa of BC043404

Sequence: ILKEITGSSLIQKTDSDEEVAMLLDTVQKVFQKMLECIARSFRKQPEEGLRLLYSVQRPLHEFITAVQSRHTDTPVHRGVLSTLIAGPVVEISHQLRKVSDVEELTPPEHLSDLPPFSRCLIGIIIKSSNVVRSFLDELKACVASNDIEGIVCLTAAVHIILVINAGKHKSSKVREVAATVHRKLKTFMEITLEEDSIERFLYESSSRTLGELLNS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 non-SMC condensin II complex, subunit G2
别名 CAP-G2, Chromosome-associated protein G2, Condensin-2 complex subunit G2, hCAP-G2, Leucine zipper protein 5
计算分子量 1143 aa, 131 kDa
观测分子量130 kDa
GenBank蛋白编号BC043404
基因名称 NCAPG2
Gene ID (NCBI) 54892
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ86XI2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NCAPG2 (non-SMC condensin II complex subunit G2) encodes a protein belonging to the non-SMC (structural maintenance protein) subunit family of the Condensin II complex that plays a key role in chromosome condensation and segregation during mitosis. NCAPG2 is positively correlated with cancer stem cell (CSC) markers, and aberrant NCAPG2 expression contributes to tumorigenesis in several human malignancies, such as prostate cancer and low-grade gliomas.

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