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NCAM2 Polyclonal antibody

NCAM2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat No : 13850-1-AP

Print datasheet

Synonyms

N CAM 2, NCAM 2, NCAM2, NCAM21

验证数据展示

  • WB analysis using 13850-1-AP

    WB analysis using 13850-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13850-1-AP (NCAM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 13850-1-AP

    IP experiment of mouse brain using 13850-1-AP

    IP result of anti-NCAM2 (IP:13850-1-AP, 3ug; Detection:13850-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • IHC staining of mouse brain using 13850-1-AP

    IHC staining of mouse brain using 13850-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13850-1-AP

    IHC staining of mouse brain using 13850-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

13850-1-AP targets NCAM2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen NCAM2 fusion protein Ag4821 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neural cell adhesion molecule 2
Synonyms N CAM 2, NCAM 2, NCAM2, NCAM21
Calculated Molecular Weight 93 kDa
Observed Molecular Weight 125 kDa
GenBank Accession NumberBC052946
Gene Symbol NCAM2
Gene ID (NCBI) 4685
RRIDAB_2237029
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15394
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NCAM2 antibody 13850-1-APDownload protocol
IHC protocol for NCAM2 antibody 13850-1-APDownload protocol
IP protocol for NCAM2 antibody 13850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

J Comp Neurol

Illuminating the terminal nerve: Uncovering the link between GnRH-1 neuron and olfactory development

Authors - Enrico Amato
View Article
mouseIHC

bioRxiv

Illuminating the Terminal Nerve: Uncovering the Link between GnRH-1 and Olfactory Development

Authors - Enrico Amato
View Article