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验证数据展示

  • WB analysis using 66088-1-Ig (same clone as 66088-1-PBS)

    WB analysis using 66088-1-Ig (same clone as 66088-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

  • IHC staining of human cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

    IHC staining of human cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 66088-1-Ig(NCALD antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66088-1-Ig (same clone as 66088-1-PBS)

    IF Staining of HeLa using 66088-1-Ig (same clone as 66088-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66088-1-Ig (NCALD antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

NCALD Monoclonal antibody, PBS Only

NCALD Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66088-1-PBS

产品说明书

CloneNo. 6B4E10

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, pig, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

NCALD, neurocalcin delta, neurocalcin δ

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66088-1-PBS targets NCALD in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, pig, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, pig, rat
免疫原

CatNo: Ag18760

Product name: Recombinant human NCALD protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-193 aa of BC036098

Sequence: MGKQNSKLRPEVMQDLLESTDFTEHEIQEWYKGFLRDCPSGHLSMEEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFIIALSVTSRGKLEQKLKWAFSMYDLDGNGYISKAEMLEIVQAIYKMVSSVMKMPEDESTPEKRTEKIFRQMDTNRDGKLSMEEFIRGAKSDPSIVRLLQCDPSSAGQF

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 neurocalcin delta
别名 NCALD, neurocalcin delta, neurocalcin δ
计算分子量 193 aa, 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC036098
基因名称 NCALD
Gene ID (NCBI) 83988
RRIDAB_11232229
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP61601
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NCALD(Neurocalcin-delta) is a member of the EF-hand calcium-binding protein superfamily.It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation.It is a high-affinity Ca2+ sensor (Kd of approx. 0.6 uM), which is most abundantly expressed in the Purkinje cells of the cerebellum(PMID:11964161).This antibody is specific to NCALD.

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