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验证数据展示

  • WB analysis of mouse colon using 12069-1-AP

    WB analysis of mouse colon using 12069-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 12069-1-AP (NBL1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 12069-1-AP

    IF Staining of HeLa using 12069-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NBL1 antibody (12069-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NBL1 Polyclonal antibody

NBL1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 12069-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

D1S1733E, DAN, DAND1, NB, Neuroblastoma suppressor of tumorigenicity 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse colon tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12069-1-AP targets NBL1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2706

Product name: Recombinant human NBL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 24-180 aa of BC012037

Sequence: LALFPDKSAWCEAKNITQIVGHSGCEAKSIQNRACLGQCFSYSVPNTFPQSTESLVHCDSCMPAQSMWEIVTLECPGHEEVPRVDKLVEKILHCSCQACGKEPSHEGLSVYVQGEDGPGSQPGTHPHPHPHPHPGGQTPEPEDPPGAPHTEEEGAED

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuroblastoma, suppression of tumorigenicity 1
别名 D1S1733E, DAN, DAND1, NB, Neuroblastoma suppressor of tumorigenicity 1
计算分子量 180 aa, 19 kDa
观测分子量 19 kDa
GenBank蛋白编号BC012037
基因名称 NBL1
Gene ID (NCBI) 4681
RRIDAB_10638604
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP41271
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NBL1 (neuroblastoma suppression of tumorigenicity 1), also named as DAN, is the founding member of the DAN subfamily. It was originally identified as a putative tumor suppressor gene in a transformed fibroblast rat model (PMID: 9516839), whereby it was significantly down-regulated in v-src-transformed rat fibroblasts. The MW of this protein is 19 kDa, and this antibody specially recognises the 19 kDa protein.

实验方案

Product Specific Protocols
IF protocol for NBL1 antibody 12069-1-APDownload protocol
WB protocol for NBL1 antibody 12069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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