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NBAS Polyclonal antibody

NBAS Polyclonal Antibody for IF/ICC, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14683-1-AP

Print datasheet

Synonyms

NAG, NBAS

验证数据展示

  • IHC staining of mouse brain using 14683-1-AP

    IHC staining of mouse brain using 14683-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14683-1-AP (NBAS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 14683-1-AP

    IHC staining of mouse brain using 14683-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14683-1-AP (NBAS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 14683-1-AP

    IF Staining of SH-SY5Y using 14683-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 14683-1-AP (NBAS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14683-1-AP targets NBAS in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC,ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen NBAS fusion protein Ag6407 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neuroblastoma amplified sequence
Synonyms NAG, NBAS
Calculated Molecular Weight 269 kDa
Observed Molecular Weight 220 kDa
GenBank Accession NumberBC051792
Gene Symbol NBAS
Gene ID (NCBI) 51594
RRIDAB_1959590
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA2RRP1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NBAS, also known as neuroblastoma amplified (NAG) gene, was identified as a gene that is co-amplified with the N-myc (MYCN) gene in neuroblastoma cell lines. NBAS is a factor of the nonsense-mediated mRNA decay (NMD), which is a highly conserved surveillance pathway that allows the organism to respond to cellular and environmental stress.(PMID: 23828042)

实验方案

Product Specific Protocols
IHC protocol for NBAS antibody 14683-1-APDownload protocol
IF protocol for NBAS antibody 14683-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Bone

Mutations in the Neuroblastoma Amplified Sequence gene in a family affected by Acrofrontofacionasal Dysostosis type 1.

Authors - Eleonora Palagano
View Article
humanIF

J Biol Chem

Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis

Authors - Robert S Kiss
View Article