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验证数据展示

  • IHC staining of mouse brain using 14683-1-AP

    IHC staining of mouse brain using 14683-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14683-1-AP (NBAS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 14683-1-AP

    IHC staining of mouse brain using 14683-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14683-1-AP (NBAS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 14683-1-AP

    IF Staining of SH-SY5Y using 14683-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 14683-1-AP (NBAS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

NBAS Polyclonal antibody

NBAS Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 14683-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA and More (2)

别名

Neuroblastoma-amplified sequence, Neuroblastoma-amplified gene protein, NBAS subunit of NRZ tethering complex, NAG

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14683-1-AP targets NBAS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 NBAS fusion protein Ag6407 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuroblastoma amplified sequence
别名 Neuroblastoma-amplified sequence, Neuroblastoma-amplified gene protein, NBAS subunit of NRZ tethering complex, NAG
计算分子量 269 kDa
观测分子量 220 kDa
GenBank蛋白编号BC051792
基因名称 NBAS
Gene ID (NCBI) 51594
RRIDAB_1959590
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA2RRP1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NBAS, also known as neuroblastoma amplified (NAG) gene, was identified as a gene that is co-amplified with the N-myc (MYCN) gene in neuroblastoma cell lines. NBAS is a factor of the nonsense-mediated mRNA decay (NMD), which is a highly conserved surveillance pathway that allows the organism to respond to cellular and environmental stress.(PMID: 23828042)

实验方案

Product Specific Protocols
IHC protocol for NBAS antibody 14683-1-APDownload protocol
IF protocol for NBAS antibody 14683-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Bone

Mutations in the Neuroblastoma Amplified Sequence gene in a family affected by Acrofrontofacionasal Dysostosis type 1.

Authors - Eleonora Palagano
View Article
humanIF

J Biol Chem

Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis

Authors - Robert S Kiss
View Article