验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HL-60 cells, human brain tissue, HeLa cells, MG-63 cells, U2OS cells |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
15807-1-AP targets NAT5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag8541 Product name: Recombinant human NAT5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-178 aa of BC005181 Sequence: MTTLRAFTCDDLFRFNNINLDPLTETYGIPFYLQYLAHWPEYFIVAEAPGGELMGYIMGKAEGSVAREEWHGHVTALSVAPEFRRLGLAAKLMELLEEISERKGGFFVDLFVRVSNQVAVNMYKQLGYSVYRTVIEYYSASNGEPDEDAYDMRKALSRDTEKKSIIPLPHPVRPEDIE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | N-acetyltransferase 5 (GCN5-related, putative) |
| 别名 | EC:2.3.1.254, Methionine N-acetyltransferase, N acetyltransferase 5, NAA20, N-acetyltransferase 5 |
| 计算分子量 | 178 aa, 20 kDa |
| 观测分子量 | 18-20 kDa |
| GenBank蛋白编号 | BC005181 |
| 基因名称 | NAT5 |
| Gene ID (NCBI) | 51126 |
| RRID | AB_1640056 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P61599 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
N-acetyltransferase 5 (NAT5/hNaa50p), also known as NAA20 (N-alpha-acetyltransferase 20), is a catalytic subunit of the N-terminal acetyltransferase B complex, which is a member of the GCN5-related N-acetyltransferase (GNAT) superfamily. It specifically acetylates the N-terminus of proteins (N-terminal acetylation) and is crucial for cell survival, chromosome segregation, and cell cycle progression. Naa20 expression is much higher in HCC tumors than in normal tissues, promoting oncogenic properties in HCC cells. Knockdown of NAA20 significantly inhibited TNBC cell viability by CCK8 and colony formation assays and cell migration and invasion (PMID: 37827343, 33219302).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NAT5 antibody 15807-1-AP | Download protocol |
| IHC protocol for NAT5 antibody 15807-1-AP | Download protocol |
| IP protocol for NAT5 antibody 15807-1-AP | Download protocol |
| WB protocol for NAT5 antibody 15807-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci NatB Catalytic Subunit Depletion Disrupts DNA Replication Initiation Leading to Senescence in MEFs | ||
Oncotarget NatB-mediated protein N-α-terminal acetylation is a potential therapeutic target in hepatocellular carcinoma. | ||
Mol Cell Biol NatB Protects Procaspase-8 from UBR4-Mediated Degradation and Is Required for Full Induction of the Extrinsic Apoptosis Pathway |
