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验证数据展示

  • WB analysis of K-562 using 16120-1-AP

    WB analysis of K-562 using 16120-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16120-1-AP (NAT13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 16120-1-AP

    IHC staining of human ovary cancer using 16120-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16120-1-AP (NAT13 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NAT13 Polyclonal antibody

NAT13 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16120-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

MAK3, hSAN, hNAT5, hNaa50p, EC:2.3.1.258

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16120-1-AP targets NAT13 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9075

Product name: Recombinant human NAT13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-165 aa of BC012731

Sequence: MKGSRIELGDVTPHNIKQLKRLNQVIFPVSYNDKFYKDVLEVGELAKLAYFNDIAVGAVCCRVDHSQNQKRLYIMTLGCLAPYRRLGIGTKMLNHVLNICEKDGTFDNIYLHVQISNESAIDFYRKFGFEIIETKKNYYKRIEPADAHVLQKNLKVPSGQNADVQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-acetyltransferase 13 (GCN5-related)
别名 MAK3, hSAN, hNAT5, hNaa50p, EC:2.3.1.258
计算分子量 169 aa, 19 kDa
观测分子量 19 kDa
GenBank蛋白编号BC012731
基因名称 NAT13
Gene ID (NCBI) 80218
RRIDAB_2148952
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9GZZ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NAT13 antibody 16120-1-APDownload protocol
IHC protocol for NAT13 antibody 16120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols