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NAT12 Polyclonal antibody

NAT12 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25149-1-AP

Print datasheet

Synonyms

C14orf35, MAK3, Mak3p, N acetyltransferase 12, NAA30, NAT12

验证数据展示

  • WB analysis using 25149-1-AP

    WB analysis using 25149-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25149-1-AP (NAT12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 25149-1-AP

    IF Staining of U2OS using 25149-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NAT12 antibody (25149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IF detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25149-1-AP targets NAT12 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human
Immunogen NAT12 fusion protein Ag18435 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-acetyltransferase 12 (GCN5-related, putative)
Synonyms C14orf35, MAK3, Mak3p, N acetyltransferase 12, NAA30, NAT12
Calculated Molecular Weight 362 aa, 39 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC118589
Gene Symbol NAT12
Gene ID (NCBI) 122830
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ147X3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

N-terminal acetylation (Nt-acetylation) by N-terminal acetyltransferases (NATs) is one of the most common protein modifications in eukaryotes. The NatC complex represents one of three major NATs of which the substrate profile remains largely unexplored. NAT12/NAA30 (N-alpha-acetyltransferase 30) is the catalytic subunit of the NAT-C complex (PMID: 27694331, 26292663). The expected sizes of the two isoforms of the protein were 35 and 39 kDa. Western blots detected two bands of NAA12, the expected ~40 kDa band and a possibly dimer at ~70 kDa.

实验方案

Product Specific Protocols
WB protocol for NAT12 antibody 25149-1-APDownload protocol
IF protocol for NAT12 antibody 25149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols