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验证数据展示

  • WB analysis using 25149-1-AP

    WB analysis using 25149-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25149-1-AP (NAT12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 25149-1-AP

    IF Staining of U2OS using 25149-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NAT12 antibody (25149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

NAT12 Polyclonal antibody

NAT12 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25149-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

C14orf35, MAK3, Mak3p, N acetyltransferase 12, NAA30, NAT12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25149-1-AP targets NAT12 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 NAT12 fusion protein Ag18435 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-acetyltransferase 12 (GCN5-related, putative)
别名 C14orf35, MAK3, Mak3p, N acetyltransferase 12, NAA30, NAT12
计算分子量 362 aa, 39 kDa
观测分子量40 kDa
GenBank蛋白编号BC118589
基因名称 NAT12
Gene ID (NCBI) 122830
RRIDAB_3085773
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ147X3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

N-terminal acetylation (Nt-acetylation) by N-terminal acetyltransferases (NATs) is one of the most common protein modifications in eukaryotes. The NatC complex represents one of three major NATs of which the substrate profile remains largely unexplored. NAT12/NAA30 (N-alpha-acetyltransferase 30) is the catalytic subunit of the NAT-C complex (PMID: 27694331, 26292663). The expected sizes of the two isoforms of the protein were 35 and 39 kDa. Western blots detected two bands of NAA12, the expected ~40 kDa band and a possibly dimer at ~70 kDa.

实验方案

Product Specific Protocols
WB protocol for NAT12 antibody 25149-1-APDownload protocol
IF protocol for NAT12 antibody 25149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols