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CoraLite® Plus 488-conjugated NAT10 Recombinant antibody

NAT10 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5B9

Cat No : CL488-82585

Print datasheet

Synonyms

ALP, DKFZp434C116, FLJ10774, FLJ12179, FLJ23850, KIAA1709, N acetyltransferase 10, NAT10

验证数据展示

  • IF Staining of HeLa using CL488-82585

    IF Staining of HeLa using CL488-82585

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 NAT10 antibody (CL488-82585, Clone: 5B9 ) at dilution of 1:800.

经过测试的应用

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 82585-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82585 targets NAT10 in IF applications and shows reactivity with Human samples.

Tested Applications IF/ICC
Tested Reactivity Human
Immunogen NAT10 fusion protein Ag4184 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name N-acetyltransferase 10 (GCN5-related)
Synonyms ALP, DKFZp434C116, FLJ10774, FLJ12179, FLJ23850, KIAA1709, N acetyltransferase 10, NAT10
Calculated Molecular Weight 1025 aa, 116 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC035558
Gene Symbol NAT10
Gene ID (NCBI) 55226
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H0A0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. The specificity of this antibody has been tested by siRNA (PMID: 24786082). NAT10 regulates mitotic cell fate by acetylating Eg5. NAT10 depletion results in multinuclear giant cells, which is the hallmark of mitotic catastrophe (PMID: 35210604). NAT10 plays a crucial role in carcinogenesis through influencing EMT, hypoxia, ribosomal biogenesis and overall, promoting translational efficiency. Recently, we reported that treating cancer cells with Remodelin, a small molecule inhibitor of NAT10, causes alteration in global lipid metabolism (PMID: 36149760).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NAT10 antibody CL488-82585Download protocol
Standard Protocols
Click here to view our Standard Protocols