验证数据展示

WB analysis of HeLa using 13365-1-AP
WB result of NAT10 antibody (13365-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected HeLa cells.
WB analysis using 13365-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13365-1-AP (NAT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 13365-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13365-1-AP (NAT10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IF Staining of NIH/3T3 using 13365-1-AP
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using NAT10 antibody (13365-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IP experiment of mouse testis using 13365-1-AP
IP result of anti-NAT10 (IP:13365-1-AP, 4ug; Detection:13365-1-AP 1:8000) with mouse testis tissue lysate 1120 ug.
IHC staining of human ovary cancer using 13365-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13365-1-AP (NAT10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 13365-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13365-1-AP (NAT10 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 13365-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (13365-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

Featured Product
KD/KO验证

NAT10 Polyclonal antibody

NAT10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13365-1-AP

产品说明书

引用文献(62)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (2)

应用

WB, IHC, IF/ICC, IP, ELISA and More (4)

别名

18S rRNA cytosine acetyltransferase, ALP, EC:2.3.1.-, KIAA1709, N acetyltransferase 10

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格:

-/ -

点击咨询售前客服

点击咨询售后客服

概述

经过测试的应用
产品信息
发表文章 (62)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A549 cells, HeLa cells, NIH/3T3 cells, Calu-1 cells
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human ovary cancer tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells, HeLa cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 13 publications below
WB	See 50 publications below
IHC	See 10 publications below
IF	See 14 publications below
IP	See 3 publications below
CoIP	See 4 publications below
ChIP	See 1 publications below
RIP	See 6 publications below

产品信息

13365-1-AP targets NAT10 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, ChIP, RIP
经测试反应性	human, mouse
文献引用反应性	human, mouse, rat, zebrafish
免疫原	NAT10 fusion protein Ag4184 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	N-acetyltransferase 10 (GCN5-related)
别名	18S rRNA cytosine acetyltransferase, ALP, EC:2.3.1.-, KIAA1709, N acetyltransferase 10
计算分子量	1025 aa, 116 kDa
观测分子量	116 kDa
GenBank蛋白编号	BC035558
基因名称	NAT10
Gene ID (NCBI)	55226
RRID	AB_2148944
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q9H0A0
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. The specificity of this antibody has been tested by siRNA. (24786082)

实验方案

Product Specific Protocols
WB protocol for NAT10 antibody 13365-1-AP	Download protocol
IHC protocol for NAT10 antibody 13365-1-AP	Download protocol
IF protocol for NAT10 antibody 13365-1-AP	Download protocol
IP protocol for NAT10 antibody 13365-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB,IP	Cell Acetylation of Cytidine in mRNA Promotes Translation Efficiency. Authors - Daniel Arango KO Validated View Article
human	WB,IF	Science Chemical inhibition of NAT10 corrects defects of laminopathic cells. Authors - Delphine Larrieu KD Validated View Article
human	WB,IP	Nat Commun N-acetyltransferase 10 is implicated in the pathogenesis of cycling T cell-mediated autoimmune and inflammatory disorders in mice Authors - Wen-Ping Li View Article
human	WB	Cell Host Microbe Acetylation of Cytidine Residues Boosts HIV-1 Gene Expression by Increasing Viral RNA Stability. Authors - Kevin Tsai KO Validated View Article
human	WB	Mol Cell Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine. Authors - Daniel Arango View Article
mouse	WB	Nat Commun Targeting of NAT10 enhances healthspan in a mouse model of human accelerated aging syndrome. Authors - Gabriel Balmus KO Validated View Article

验证数据展示

WB analysis of HeLa using 13365-1-AP

WB analysis using 13365-1-AP

WB analysis using 13365-1-AP

IF Staining of NIH/3T3 using 13365-1-AP

IP experiment of mouse testis using 13365-1-AP

IHC staining of human ovary cancer using 13365-1-AP

IHC staining of human stomach cancer using 13365-1-AP

IF Staining of HeLa using 13365-1-AP

NAT10 Polyclonal antibody

NAT10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Acetylation of Cytidine in mRNA Promotes Translation Efficiency.

Chemical inhibition of NAT10 corrects defects of laminopathic cells.

N-acetyltransferase 10 is implicated in the pathogenesis of cycling T cell-mediated autoimmune and inflammatory disorders in mice

Acetylation of Cytidine Residues Boosts HIV-1 Gene Expression by Increasing Viral RNA Stability.

Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine.

Targeting of NAT10 enhances healthspan in a mouse model of human accelerated aging syndrome.