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NAT10 Polyclonal antibody
NAT10 Polyclonal Antibody for WB, IP, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF/ICC, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells, NIH/3T3 cells |
Positive IP detected in | mouse testis tissue |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13365-1-AP targets NAT10 in WB, IP, IF, RIP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, IF/ICC, ELISA |
Cited Applications | WB, IP, IF, RIP, IHC, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | NAT10 fusion protein Ag4184 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | N-acetyltransferase 10 (GCN5-related) |
Synonyms | ALP, KIAA1709, N acetyltransferase 10, NAT10, NET43 |
Calculated Molecular Weight | 1025 aa, 116 kDa |
Observed Molecular Weight | 116 kDa |
GenBank Accession Number | BC035558 |
Gene Symbol | NAT10 |
Gene ID (NCBI) | 55226 |
RRID | AB_2148944 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9H0A0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for NAT10 antibody 13365-1-AP | Download protocol |
IF protocol for NAT10 antibody 13365-1-AP | Download protocol |
IP protocol for NAT10 antibody 13365-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Host Microbe Acetylation of Cytidine Residues Boosts HIV-1 Gene Expression by Increasing Viral RNA Stability.
| ||
Mol Cell Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine. | ||
Nat Commun Targeting of NAT10 enhances healthspan in a mouse model of human accelerated aging syndrome.
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Nucleic Acids Res NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells. |