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验证数据展示

  • WB analysis using 15714-1-AP

    WB analysis using 15714-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15714-1-AP (NARS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 15714-1-AP

    WB analysis of HepG2 using 15714-1-AP

    WB result of NARS2 antibody (15714-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NARS2 transfected HepG2 cells.

  • IHC staining of human kidney using 15714-1-AP

    IHC staining of human kidney using 15714-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15714-1-AP (NARS2 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with NARS2 antibody included
  • IHC staining of human kidney using 15714-1-AP

    IHC staining of human kidney using 15714-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15714-1-AP (NARS2 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with NARS2 antibody included
  • IF Staining of HepG2 using 15714-1-AP

    IF Staining of HepG2 using 15714-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15714-1-AP (NARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

NARS2 Polyclonal antibody

NARS2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15714-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

AsnRS, Asparagine tRNA ligase, NARS2, SLM5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, human liver tissue, human brain tissue, K-562 cells, mouse kidney tissue
Positive IHC detected inhuman kidney tissue, human heart tissue, human lung tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NARS2 Ready-To-Use IHC Kit. NARS2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15714-1-AP targets NARS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8345

Product name: Recombinant human NARS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 132-477 aa of BC007800

Sequence: ERHPLEYLRQYPHFRCRTNVLGSILRIRSEATAAIHSFFKDSGFVHIHTPIITSNDSEGAGELFQLEPSGKLKVPEENFFNVPAFLTVSGQLHLEVMSGAFTQVFTFGPTFRAENSQSRRHLAEFYMIEAEISFVDSLQDLMQVIEELFKATTMMVLSKCPEDVELCHKFIAPGQKDRLEHMLKNNFLIISYTEAVEILKQASQNFTFTPEWGADLRTEHEKYLVKHCGNIPVFVINYPLTLKPFYMRDNEDGPQHTVAAVDLLVPGVGELFGGGLREERYHFLEERLARSGLTEVYQWYLDLRRFGSVPHGGFGMGFERYLQCILGVDNIKDVIPFPRFPHSCLL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 asparaginyl-tRNA synthetase 2, mitochondrial (putative)
别名 AsnRS, Asparagine tRNA ligase, NARS2, SLM5
计算分子量 477 aa, 54 kDa
观测分子量 50-54 kDa
GenBank蛋白编号BC007800
基因名称 NARS2
Gene ID (NCBI) 79731
RRIDAB_2151157
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96I59
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for NARS2 antibody 15714-1-APDownload protocol
IHC protocol for NARS2 antibody 15714-1-APDownload protocol
WB protocol for NARS2 antibody 15714-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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