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NARS Monoclonal antibody

NARS Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

4G5G12

Cat No : 67711-1-Ig

Print datasheet

Synonyms

ASNRS, Asparagine tRNA ligase, asparaginyl tRNA synthetase, NARS, NARS1

验证数据展示

  • WB analysis using 67711-1-Ig

    WB analysis using 67711-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67711-1-Ig (NARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67711-1-Ig

    IF Staining of HeLa using 67711-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NARS antibody (67711-1-Ig, Clone: 4G5G12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human urothelial carcinoma using 67711-1-Ig

    IHC staining of human urothelial carcinoma using 67711-1-Ig

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67711-1-Ig (NARS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NARS antibody included

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NARS Ready-To-Use IHC Kit. NARS primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67711-1-Ig targets NARS in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen NARS fusion protein Ag6656 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name asparaginyl-tRNA synthetase
Synonyms ASNRS, Asparagine tRNA ligase, asparaginyl tRNA synthetase, NARS, NARS1
Calculated Molecular Weight 63 kDa
Observed Molecular Weight63 kDa
GenBank Accession NumberBC001687
Gene Symbol NARS
Gene ID (NCBI) 4677
RRIDAB_2882901
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43776
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Asparagine-tRNA ligase (NARS) belongs to the class-II aminoacyl-tRNA synthetase family. Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn) (PubMed: 9421509). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells. The NARS levels were higher in tumor tissues compared with adjacent normal tissues, and positively associated with LN metastasis. NARS may serve as a potential biomarker for lung adenocarcinoma (ADC) diagnosis/prognosis (PMID: 27161446).

实验方案

Product Specific Protocols
WB protocol for NARS antibody 67711-1-IgDownload protocol
IHC protocol for NARS antibody 67711-1-IgDownload protocol
IF protocol for NARS antibody 67711-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols